TLR8 Antibody (YA7757)(PBS only)

Cat. No.: HY-P88073A: Data Sheet User Guide for Antibodies
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TLR8 Antibody (YA7757) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to TLR8.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

TLR8 Antibody (YA7757) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to TLR8.

Host

Mouse

Molecular Weight

Predicted band size: 119.6 kDa

Species Reactivity

Human

SwissProt ID

Q9NR97

Immunogen

Human recombinant protein fragment corresponding to amino acids 32-153 of human TLR8 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:2000-2500

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Endosomal receptor that plays a key role in innate and adaptive immunity (PubMed:25297876, PubMed:32433612). Controls host immune response against pathogens through recognition of RNA degradation products specific to microorganisms that are initially processed by RNASET2 (PubMed:31778653). Recognizes GU-rich single-stranded RNA (GU-rich RNA) derived from SARS-CoV-2, SARS-CoV-1 and HIV-1 viruses (PubMed:33718825). Upon binding to agonists, undergoes dimerization that brings TIR domains from the two molecules into direct contact, leading to the recruitment of TIR-containing downstream adapter MYD88 through homotypic interaction (PubMed:23520111, PubMed:25599397, PubMed:26929371, PubMed:33718825). In turn, the Myddosome signaling complex is formed involving IRAK4, IRAK1, TRAF6, TRAF3 leading to activation of downstream transcription factors NF-kappa-B and IRF7 to induce pro-inflammatory cytokines and interferons, respectively (PubMed:16737960, PubMed:17932028, PubMed:29155428)

Subcellular Localization：Endosome membrane

Expression：
Tissue_Specificity: Expressed in myeloid dendritic cells, monocytes, and monocyte-derived dendritic cells

Isoforms & Post-Translational Modification：Q9NR97 has two isomers: Q9NR97-1: 119828 Da (predicted); Q9NR97-2: 121764 Da (predicted).
Ubiquitinated by RNF216; leading to degradation by the proteasome丨Proteolytic processing occurs in monocytes and monocyte-derived macrophages by both furin-like proprotein convertase and cathepsins (PubMed:25297876)

Subunit：Homodimer (PubMed:23520111, PubMed:25599397, PubMed:26929371, PubMed:29155428)

Synonyms

CD288

Documentation

User Guide for Antibodies (1077 KB)