Apoptosis inducer 59
Apoptosis inducer 59, a camphorsulfonamide-based thiazolylhydrazone derivative, is a apoptosis inducer with antitumor activity. Apoptosis inducer 59 can inhibit cell migration and invasiveness, cause S phase arrest, induce ROS production and depolarise mitochondrial membrane potential. Apoptosis inducer 59 can be used for the research of cancer, such as breast cancer.
For research use only. We do not sell to patients.
- Formula: C25H27ClN4O3S2
- Molecular Weight:531.09
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Apoptosis inducer 59 (Compound 5J) (48 h) potently and selectively inhibits MCF-7 breast cancer cell proliferation with an IC50 of 12.80 ± 0.12 μM, while exhibiting low cytotoxicity to normal LO2 liver cells (IC50 >50 μM)[1].
Apoptosis inducer 59 (5-25 μM; 24 h) dose-dependently induces apoptosis in MCF-7 breast cancer cells, with a combined early and late apoptosis rate of 25.52%[1].
Apoptosis inducer 59 (5-25 μM; 24 h) induces S phase cell cycle arrest in MCF-7 breast cancer cells, increasing the S phase population from 21.78% to 31.32%[1].
Apoptosis inducer 59 (5-25 μM; 24-48 h) inhibits MCF-7 breast cancer cell migration and invasiveness[1].
Apoptosis inducer 59 (5-25 μM) dose-dependently increases intracellular ROS production in MCF-7 breast cancer cells[1].
Apoptosis inducer 59 (5-25 μM) concentration-dependently depolarizes the mitochondrial membrane potential in MCF-7 breast cancer cells, leading to mitochondrial dysfunction[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MCF-7 breast cancer cells
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Concentration:5, 10 and 25 μM
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Incubation Time:24 h
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Result:Increased apoptosis rates in MCF-7 cells
(10.62%, 11.60%, 18.17%).
Showed early and late apoptosis rate of 25.52% higher than the control group (9.50%).
- Scratch/Wound-Healing Migration Assay
- Mitochondrial membrane-potential fluorescent assay
- Annexin V plus membrane-impermeant dye apoptosis staining
- Cell migration
- BrdU Incorporation Assay
- ROS/oxidative-stress fluorescent staining
- Neural Crest/Neuronal Cell Migration Explant Assay
- Protocol for Cell Cycle
- Breast Cancer Modeling
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Flow cytometric DNA-content cell-cycle staining
- Zigmond Chamber Chemotaxis Assay
- Under-Agarose Cell Migration Assay
- Transwell/Boyden Chamber Migration Assay
- Fluorescent plasma-membrane potential dye assay
- Mitochondrial membrane-potential and mitochondrial mass staining
- Apoptosis Solutions
- 3D Collagen/Hydrogel Matrix Migration Assay
- Subcutaneous Cell-Line-Derived Xenograft
- Cell-Exclusion Zone Migration Assay
- Dunn/Insall Chamber Chemotaxis Assay
Chemical Information
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Molecular Weight 531.09
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Formula C25H27ClN4O3S2
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SMILES
O=S(CC1(C2(C)C)/C(CC2CC1)=N/NC3=NC(C4=CC=C(O)C=C4)=CS3)(NC5=CC=CC(Cl)=C5)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Apoptosis inducer 59 Related Classifications
HY-181274 Related Classifications
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