Br-DAPI

Name: Br-DAPI
Brand: MedChemExpress
SKU: HY-D1396
Rating: 5.0 (3 reviews)

Cat. No.: HY-D1396 Purity: 98.0%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Br-DAPI is a marker dye in DAPI series. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. In addition, because DAPI can pass through intact cell membranes, it can be used to stain both live and fixed cells. Storage: Keep away from light.

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Br-DAPI 화학구조

CAS No. : 2387906-44-5

사이즈	재고	수량

무료 샘플 (0.1 - 0.2 mg)	지금 신청하기
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
25 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	해외재고보유	Estimated Time of Arrival: December 31
100 mg	해외재고보유	Estimated Time of Arrival: December 31
200 mg	견적 받기
500 mg	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 3 publication(s) in Google Scholar

3 Publications Citing Use of MCE Br-DAPI

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•Related Small Molecules:

•Related Proteins:

Biological Activity
순도&문서
References
고객리뷰

제품 설명

Cellular Effect

Cell Line	Type	Value	Description	References
MCF7	CC₅₀	7.6 3 Compound: Br-DAPI	Photocytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 1 hr followed by washout and irradiation with 8 J/cm^2 UV irradiation for 5 mins and measured after 24 hrs by MTT assay Photocytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 1 hr followed by washout and irradiation with 8 J/cm^2 UV irradiation for 5 mins and measured after 24 hrs by MTT assay	[PMID: 36480920]
MCF7	CC₅₀	> 64 3 Compound: Br-DAPI	Photocytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 1 hr under dark condition followed by washout and measured after 24 hrs by MTT assay Photocytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 1 hr under dark condition followed by washout and measured after 24 hrs by MTT assay	[PMID: 36480920]
MCF7	CC₅₀	7.6 3 Compound: Br-DAPI	Photocytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 1 hr followed by washout and irradiation with 8 J/cm^2 UV irradiation for 5 mins and measured after 24 hrs by MTT assay Photocytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 1 hr followed by washout and irradiation with 8 J/cm^2 UV irradiation for 5 mins and measured after 24 hrs by MTT assay	[PMID: 36480920]
MCF7	CC₅₀	7.6 3 Compound: Br-DAPI	Photocytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 1 hr followed by washout and irradiation with 8 J/cm^2 UV irradiation for 5 mins and measured after 24 hrs by MTT assay Photocytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 1 hr followed by washout and irradiation with 8 J/cm^2 UV irradiation for 5 mins and measured after 24 hrs by MTT assay	[PMID: 36480920]
MCF7	CC₅₀	>64 3 Compound: Br-DAPI	Photocytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 1 hr under dark condition followed by washout and measured after 24 hrs by MTT assay Photocytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 1 hr under dark condition followed by washout and measured after 24 hrs by MTT assay	[PMID: 36480920]
MCF7	CC₅₀	> 64 3 Compound: Br-DAPI	Photocytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 1 hr under dark condition followed by washout and measured after 24 hrs by MTT assay Photocytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 1 hr under dark condition followed by washout and measured after 24 hrs by MTT assay	[PMID: 36480920]

In Vitro

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Preparation of DAPI working solution
1.1 Preparation of the stock solution
Dissolve 1 mg DAPI in 1 mL ddH₂O to obtain 1 mg/mL of stock solution.
Note: It is recommended to store the stock solution at -20°C or -80°C away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of DAPI working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 ug/mL of working solution.
Note: Please adjust the concentration of DAPI working solution according to the actual situation.
2. Cell staining
2.1 Suspension cells (6-well plate)
a. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×10⁶/mL.
b. Add 1 mL of working solution, and then incubate at room temperature for 3-10 minutes.
c. Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
d. Wash twice with PBS, 5 minutes each time.
e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 3-10 minutes.
d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry.

Precautions 1. Please adjust the concentration of DAPI working solution according to the actual situation.
2. This product is for R&D use only, not for drug, household, or other uses.
3. For your safety and health, please wear a lab coat and disposable gloves to operate.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

356.22

화학식

C₁₆H₁₄BrN₅

CAS No.

2387906-44-5

Appearance

Solid

Color

Light yellow to yellow

SMILES

N=C(C1=CC2=C(C=C1)C(Br)=C(C3=CC=C(C(N)=N)C=C3)N2)N

선적

Room temperature in continental US; may vary elsewhere.

보관

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

용액&용해도

In Vitro:

DMSO : 100 mg/mL (280.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8073 mL	14.0363 mL	28.0725 mL
5 mM	0.5615 mL	2.8073 mL	5.6145 mL
10 mM	0.2807 mL	1.4036 mL	2.8073 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

순도&문서

Purity: 98.0%

Select Batch:

Data Sheet (272 KB) SDS (252 KB)

COA (246 KB) HNMR (118 KB) LCMS (385 KB)

Handling Instructions (2659 KB)

References

[1]. Kapuscinski J, et al. DAPI: a DNA-specific fluorescent probe. Biotech Histochem. 1995 Sep;70(5):220-33. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.8073 mL	14.0363 mL	28.0725 mL	70.1813 mL
	5 mM	0.5615 mL	2.8073 mL	5.6145 mL	14.0363 mL
	10 mM	0.2807 mL	1.4036 mL	2.8073 mL	7.0181 mL
	15 mM	0.1872 mL	0.9358 mL	1.8715 mL	4.6788 mL
	20 mM	0.1404 mL	0.7018 mL	1.4036 mL	3.5091 mL
	25 mM	0.1123 mL	0.5615 mL	1.1229 mL	2.8073 mL
	30 mM	0.0936 mL	0.4679 mL	0.9358 mL	2.3394 mL
	40 mM	0.0702 mL	0.3509 mL	0.7018 mL	1.7545 mL
	50 mM	0.0561 mL	0.2807 mL	0.5615 mL	1.4036 mL
	60 mM	0.0468 mL	0.2339 mL	0.4679 mL	1.1697 mL
	80 mM	0.0351 mL	0.1755 mL	0.3509 mL	0.8773 mL
	100 mM	0.0281 mL	0.1404 mL	0.2807 mL	0.7018 mL

Br-DAPI Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Br-DAPI2387906-44-5Fluorescent DyeDNA StainInhibitorinhibitorinhibit

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Br-DAPI

고객리뷰

Br-DAPI Related Antibodies

3 Publications Citing Use of MCE Br-DAPI

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

순도&문서

References

고객리뷰

Br-DAPI Related Antibodies

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

Br-DAPI Related Classifications

Keywords:

최근 본 상품:

고객리뷰

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report