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  3. Hydrolases (EC 3)
  4. Fumarylacetoacetase/FAH Protein, Human (HEK293, His)

Fumarylacetoacetase/FAH Protein, Human (HEK293, His)

Cat. No.: HY-P70307
COA Handling Instructions

Fumarylacetoacetase/FAH Protein is a key enzyme in the metabolism of phenylalanine and tyrosine expressed primarily in the liver. Deficiency of FAH causes hereditary tyrosinemia type 1. Fumarylacetoacetase/FAH Protein, Human (HEK293, His) is the recombinant human-derived Fumarylacetoacetase/FAH protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Fumarylacetoacetase/FAH Protein, Human (HEK293, His) is 418 a.a., with molecular weight of ~43.0 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

Fumarylacetoacetase/FAH Protein is a key enzyme in the metabolism of phenylalanine and tyrosine expressed primarily in the liver. Deficiency of FAH causes hereditary tyrosinemia type 1. Fumarylacetoacetase/FAH Protein, Human (HEK293, His) is the recombinant human-derived Fumarylacetoacetase/FAH protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Fumarylacetoacetase/FAH Protein, Human (HEK293, His) is 418 a.a., with molecular weight of ~43.0 kDa.

Background

Fumarylacetoacetase is an enzyme encoded by the FAH gene on human chromosome 15. The FAH gene is believed to be involved in the catabolism of phenylalanine in humans and is a key enzyme in the metabolism of phenylalanine and tyrosine, which is mainly expressed in the liver. FAH is a protein homodimer that splits the carbon-carbon bond of fumarylacetic acid in a hydrolysis reaction. The active site of FAH contained Ca2+. Deficiency of FAH causes hereditary tyrosinemia type 1[1][2][3].

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

P16930 (S2-S419)

Gene ID
Molecular Construction
N-term
FAH (S2-S419)
Accession # P16930
6*His
C-term
Synonyms
rHuFumarylacetoacetase/FAH, His; Fumarylacetoacetase; FAA; Beta-Diketonase; Fumarylacetoacetate Hydrolase; FAH
AA Sequence

SFIPVAEDSDFPIHNLPYGVFSTRGDPRPRIGVAIGDQILDLSIIKHLFTGPVLSKHQDVFNQPTLNSFMGLGQAAWKEARVFLQNLLSVSQARLRDDTELRKCAFISQASATMHLPATIGDYTDFYSSRQHATNVGIMFRDKENALMPNWLHLPVGYHGRASSVVVSGTPIRRPMGQMKPDDSKPPVYGACKLLDMELEMAFFVGPGNRLGEPIPISKAHEHIFGMVLMNDWSARDIQKWEYVPLGPFLGKSFGTTVSPWVVPMDALMPFAVPNPKQDPRPLPYLCHDEPYTFDINLSVNLKGEGMSQAATICKSNFKYMYWTMLQQLTHHSVNGCNLRPGDLLASGTISGPEPENFGSMLELSWKGTKPIDLGNGQTRKFLLDGDEVIITGYCQGDGYRIGFGQCAGKVLPALLPS

Molecular Weight

Approximately 43.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Fumarylacetoacetase/FAH Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Fumarylacetoacetase/FAH Protein, Human (HEK293, His)
Cat. No.:
HY-P70307
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