2. Cell Cycle/DNA Damage
  3. Endonuclease
  4. Deoxyribonuclease II

Deoxyribonuclease II (DNase II) is an endonuclease that hydrolyzes the phosphodiester bonds of deoxyribonucleotides in native and denatured DNA, producing 3' phosphate and 5'-hydroxyl termini. Deoxyribonuclease II works best at acidic pH and is commonly used in biochemical research.

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Deoxyribonuclease II

Deoxyribonuclease II Chemische Struktur

CAS. Nr. : 9025-64-3

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Deoxyribonuclease II Related Antibodies

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Beschreibung

Deoxyribonuclease II (DNase II) is an endonuclease that hydrolyzes the phosphodiester bonds of deoxyribonucleotides in native and denatured DNA, producing 3' phosphate and 5'-hydroxyl termini. Deoxyribonuclease II works best at acidic pH and is commonly used in biochemical research[1].

In Vitro

Product Information
Molecular Weight: 38.0 kDa
Optimal pH: 4.5-5.0
Activators: Cysteine, EDTA
Inhibitors: Actinomycin D, iodoacetate, N-bromosuccinimide, H2O2, divalent cations (such as zinc and copper), sulfate, NaCl (above 250 mM), DNA (above 4 mg/mL)
Instructions
Soluble in pure water (1 mg/mL), fresh preparation is recommended. Or dissolve in buffer according to specific experimental references.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Deoxyribonuclease II Related Antibodies
CAS. Nr.
Appearance

Solid

Color

White to light yellow

SMILES

[Deoxyribonuclease II]
EC Number

3.1.22.1
Enzyme Activity

≥800 U/mg soild
Unit Definition

One unit is defined as the amount of enzyme that can causes an increase in absorbance at 260 nm of 0.001 per minute at 25°C, pH 4.6.
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.
Lösungsmittel & Löslichkeit
In Vitro: 

H2O : ≥ 5 mg/mL

*"≥" means soluble, but saturation unknown.

  • Molaritätsrechner

  • Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Reinheit & Dokumentation

SDS (251 KB)

COA (242 KB)

Handling Instructions (2659 KB)
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Deoxyribonuclease II Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Deoxyribonuclease II9025-64-3DNase IIEndonucleaseInhibitorinhibitorinhibit

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