Ion Fluorescent Indicators

Ions exhibit differential distribution inside and outside the cell, forming the basis for maintaining cellular electrochemical homeostasis and signal transduction. Imbalances in ion homeostasis are closely associated with various pathophysiological processes mediated by electrophysiological activity.Ion Fluorescent Probes, as highly sensitive and fast-responding detection tools, can monitor real-time changes in ion concentration and localization.They are commonly applied in studies of membrane potential dynamics, spatiotemporal ion homeostasis and related cellular processes. The fluorescent stock solutions are easy to use with minimized reagent loss, while ensuring experimental reproducibility.

Ion Fluorescent Indicators (10):

Cat. No.	Product Name	CAS No.	Purity	Chemical Structure

HY-DY1020

Rhod-2 AM (solution)	145037-81-6
Rhod-2 (solution) is a high-affinity visible light excitation wavelength Ca²⁺ fluorescent probe, Rhod-2, AM is an acetyl methyl ester derivative of Rhod-2, which has cell membrane permeability and can easily enter cells with simple culture. Once it enters the cell, it is sheared by its lactesterase to produce Rhod-2 without membrane permeability, which remains in the cell to perform the corresponding physiological functions. Maximum excitation/emission wavelength: 549/578 nm. Solvent and concentration: DMSO: 1 mM

HY-DY1030

RhoNox-1 (solution)	1447815-38-4
RhoNox-1 (solution) is a fluorescent probe for the specific detection of divalent iron ions, and when RhoNox-1 reacts with Fe²⁺. RhoNox-1 can generate an irreversible orange (red) fluorescent product (Ex/Em：540/575 nm). FeRhoNox-1 can enter the cell well, suitable for the detection of Fe²⁺ in living cells, and tends to be localized in the Golgi apparatus. Solvent and concentration: DMSO: 5 mM

HY-DY1089

Di-4-ANEPPDHQ (solution)	797785-10-5
Di-4-ANEPPDHQ (solution) is a polarity-sensitive membrane probe with a doubly positive charge that can be used to visualize membrane microdomains in living Arabidopsis cells. Di-4-ANEPPDHQ produces a fluorescence spectrum shift by changing the polarity of the lipid environment after embedding into the cell membrane, thereby quantifying the degree of lipid accumulation. The excitation wavelength of Di-4-ANEPPDHQ is 488 nm, and the emission wavelengths are 560 nm and 650 nm in the liquid ordered and disordered phases, respectively. Di-4-ANEPPDHQ is often used for fluorescence imaging studies of membrane microstructure, lipid order, and membrane tension response in living cells[1][2][3]. Solvent and concentration: DMSO: 5 mM

HY-DY1028

Fluo-4 AM (solution)	273221-67-3
Fluo-4 AM (solution) is a cell-permeable Ca²⁺ (calcium ion) indicator (Ex/Em = 485/526 nm) . Solvent and concentration: DMSO: 1 mM

HY-DY1099

7-Aminoactinomycin D (solution)	7240-37-1
7-Aminoactinomycin D (solution) (7-AAD (solution)) a cell-impermeant fluorescent DNA stain, is a potent RNA polymerase inhibitor. 7-Aminoactinomycin D selectively binds to GC regions of the DNA. 7-Aminoactinomycin D also has antibacterial effects. Solvent and concentration: DMSO: 1 mg/mL

HY-DY1095

Fluo-3AM (solution)	121714-22-5
Fluo-3AM (solution) is a fluorecent Ca²⁺ chelator, with high affinity for calcium. Fluo-3 AM can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM acetylmethyl ester can enter the cell well, after being sheared by the intracellular esterase stay in the cell to bind to calcium ions, produce strong fluorescence. Solvent and concentration: DMSO: 5 mM

HY-DY1106

Ferrozine (solution)	69898-45-9
Ferrozine (solution) is a spectrophotometric reagent for iron ions, can react with divalent Fe to form a stable magenta complex species. The complex has an absorption peak at 562 nm. Ferrozine-based colorimetric assays can quantify iron in cells Solvent and concentration: ddH2O: 10 mM

HY-DY1104

Copper Fluor-4 (solution)	2365532-64-3
Copper Fluor-4 (solution) (CF4 (solution)) is a Cu⁺-specific fluorescent probe based on a rhodol dye scaffold. Copper Fluor-4 solution has high copper selectivity with a K_d value of 2.9×10^?13 M, particularly over zinc and iron, as well as abundant cellular alkali and alkaline earth metals. Copper Fluor-4 solution is stable in a physiologically relevant pH regime between 6 and 8 (wavelengths of 415 nm for excitation and 660 nm for emission). Solvent and concentration: DMSO: 5 mM

HY-DY1083

Zinquin (solution)	151606-29-0
Zinquin (solution) is a cell-impermeant fluorescent sensor and used to observe reactive Zn²⁺. λex/λem=368/490 nm. Solvent and concentration: DMSO: 5 mM

HY-DY1055

TSQ (solution)	109628-27-5
TSQ (solution) is a cytosolic zinc ion fluorescence probe that is membrane permeable and can be used for intracellular imaging of zinc proteins (λ_max ~470 nm). TSQ can combine with Zn²⁺ in the presence of Ca²⁺ and Mg²⁺ to produce blue fluorescence (Ex/Em = 334/495 nm) . Solvent and concentration: DMSO: 10 mM

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