Endo-β-N-acetylglucosaminidase (Endo S) 

Endo-β-N-acetylglucosaminidase (Endo S) is a family 18 glycosyl hydrolase secreted by Streptococcus pyogenes. Endo-β-N-acetylglucosaminidase (Endo S) exclusively hydrolyzes the β-1,4-di-N-acetylchitobiose core of the asparagine-linked complex-type glycan on Asn-297 of the γ-chains of IgG antibody^[1].

Product Information
This product is expressed by recombinant E. coli, with N-terminal His-tag, molecular weight of 136 kDa, and buffer components of 20 mM Tris-HCl (pH7.5), 200 mM NaCl, and 10% glycerol. It can be used for antibody glycosylation modification and analysis.

Instructions
1. Add 1 μL of 500 mM sodium phosphate (pH4.5) to 10 μg bovine fibrinogen, then add Endo S and incubate at 37°C for 1 hour.
2. Analyze by SDS-PAGE or mass spectrometry.

Notes
The incubation time or enzyme amount needs to be optimized according to the actual situation.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

37278-88-9

Liquid

Colorless to light yellow

[Endo-b-N-acetylglucosaminidase(Endo S)]

One unit is defined as the amount of enzyme that more than 95% of carbohydrates were removed from 5 μg human IgG at 37°C, reacting 1 hour, in a 10 μL reaction system.

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Nandakumar KS, et, al. Streptococcal Endo-β-N-Acetylglucosaminidase Suppresses Antibody-Mediated Inflammation In Vivo. Front Immunol. 2018 Jul 16;9:1623.  [Content Brief]