Endo-β-N-acetylglucosaminidase F2  (Synonyms: Endo F2)

Endo-β-N-acetylglucosaminidase F2 (Endo F2), a highly specific endoglycosidase, cleaves within the chitobiose core of asparagine-linked complex biantennary and high mannose oligosaccharides from glycoproteins and glycopeptides. Endo F2 cleaves biantennary glycans at a rate approximately 20 times greater than high mannose glycans. The activity of Endo F2 is identical on biantennary structures with and without core fucosylation. Endo F2 is not active on hybrid or tri- and tetra-antennary oligosaccharides^[1].

others

Protocol
1) For 10 μg of glycoprotein, add 1 μl of 500 mM sodium acetate (pH 5.5), and adjust the volume to 10 μL with deionized water;
2) Add 1-2 μL of Endo F2, pipe gently to mix;
3) Incubate at 37 °C for 1 hour;
4) Analyze by SDS-PAGE or mass spectrometry;
Note: Incubation time or enzyme amount needs to be optimized according to actual conditions.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

37278-88-9

Liquid

Colorless to light yellow

[Endo-b-N-acetylglucosaminidase F2]

3.2.1.96

≥100 U/mL

One unit is defined as the amount of enzyme that more than 95% of carbohydrates were removed from denatured 10 μg Bovine blood fibrinogen at 37°C, reacting 1 hour, in a 10 μL reaction system.

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. R B Trimble, et al. Identification of distinct endoglycosidase (endo) activities in Flavobacterium meningosepticum: endo F1, endo F2, and endo F3. Endo F1 and endo H hydrolyze only high mannose and hybrid glycans. J Biol Chem. 1991 Jan 25;266(3):1646-51.  [Content Brief]