Endo H, Streptomyces picatus  (Synonyms: Endo-β-N-acetylglucosaminidase H)

Endo H, Streptomyces picatus (Endo-β-N-acetylglucosaminidase H), isolated from Streptomyces plicatus, hydrolyzes the central glycosidic bond of the β1, 4-di-N-acetylchitobiose core in asparagine-linked oligosaccharides^[1].

others

Protocol
1) For 10 μg of RNase B, add 1 μL of 500 mM sodium acetate (pH 7.5), and adjust the volume to 10 μL with deionized water;
2) Add 1-2 μL of EndoH, pipe gently to mix;
3) Incubate at 37°C for 1 hour;
4) Analyze by SDS-PAGE or mass spectrometry.
Note: Incubation time or enzyme amount needs to be optimized according to actual conditions.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

37278-88-9

Liquid

Colorless to light yellow

[Endo H, Streptomyces picatus]

3.2.1.96

≥5000 U/mg protein

One unit is defined as the amount of enzyme that split 10 μg of human RNase B > 95% of the carbohydrate in 1 hour at 37°C in 10 μL total reaction volume.

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. V Rao, et al. Crystal structure of endo-beta-N-acetylglucosaminidase H at 1.9 A resolution: active-site geometry and substrate recognition. Structure. 1995 May 15;3(5):449-57.  [Content Brief]