1. Epigenetics
  2. DNA Methyltransferase
  3. Glucose-conjugated MGMT inhibitor

Glucose-conjugated MGMT inhibitor (Synonyms: O6BTG-C8-βGlu)

Cat. No.: HY-13057
Handling Instructions

Glucose-conjugated MGMT inhibitor is a potent O6-methylguanine-DNAmethyl-transferase (MGMT) inhibitor, with IC50s of 32 nM in vitro (cell extracts) and 10 nM in HeLa S3 cells.

For research use only. We do not sell to patients.

Glucose-conjugated MGMT inhibitor Chemical Structure

Glucose-conjugated MGMT inhibitor Chemical Structure

CAS No. : 382607-78-5

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 570 In-stock
Estimated Time of Arrival: December 31
5 mg USD 420 In-stock
Estimated Time of Arrival: December 31
10 mg USD 600 In-stock
Estimated Time of Arrival: December 31
50 mg USD 1800 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 4 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Glucose-conjugated MGMT inhibitor purchased from MCE. Usage Cited in: Tag der mündlichen Prüfung. 22. Juli 2014.

    Glukose-konjugierte Hemmstoffe als Strategie der gezielten Hemmung des DNA-Reparaturproteins O6-Methylguanin-DNA-Methyltransferase in Tumorzellen und der Einfluss von ATP-binding cassette Transportern.

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    Description

    Glucose-conjugated MGMT inhibitor is a potent O6-methylguanine-DNAmethyl-transferase (MGMT) inhibitor, with IC50s of 32 nM in vitro (cell extracts) and 10 nM in HeLa S3 cells.

    IC50 & Target[1]

    MGMT

    32 nM (IC50)

    In Vitro

    Glucose-conjugated MGMT inhibitor (O6BTG-octylglucoside) is a potent and non-toxic MGMT inhibitor, with IC50s of 32 nM in vitro (cell extracts) and 10 nM in HeLa S3 cells[1].

    Molecular Weight

    616.53

    Formula

    C₂₄H₃₄BrN₅O₇S

    CAS No.

    382607-78-5

    SMILES

    [H][[email protected]]1([[email protected]]([H])(O[[email protected]@](OCCCCCCCCN2C3=C(N=C2)C(OCC4=CC(Br)=CS4)=NC(N)=N3)([[email protected]](O)([[email protected]]1(O)[H])[H])[H])CO)O

    Shipping

    Room temperature in continental US; may vary elsewhere

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (162.20 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.6220 mL 8.1099 mL 16.2198 mL
    5 mM 0.3244 mL 1.6220 mL 3.2440 mL
    10 mM 0.1622 mL 0.8110 mL 1.6220 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (4.05 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (4.05 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (4.05 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
    Kinase Assay
    [1]

    For the assays, 100 mg cell extract protein are used. In each assay, a negative and a positive sample, HeLa MR (MGMT-deficient) and HeLa S3 cell extract, respectively, are included. Incubation occurs in buffer contain-ing 700 mM HEPES-KOH (pH 7.8), 10 mM dithiothreitol and 50 mM EDTA for 90 min, which is the optimal time span for the reaction to be completed. Data are expressed as femtomoles of radioactivity trans-ferred from 3H-methylnitrosourea-labeled DNA to protein per milligram of protein within Glucose-conjugated MGMT inhibitor[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Product Name:
    Glucose-conjugated MGMT inhibitor
    Cat. No.:
    HY-13057
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