In vivo OptiLNP RNA Transfection Reagent (Local Effect) 

Lipid nanoparticles (LNP) are a lipid-based nanoscale drug delivery system widely used for the delivery of nucleic acids (such as mRNA, siRNA, DNA, etc.) and small molecule drugs.

Naked RNA is a negatively charged hydrophilic macromolecule that faces challenges in entering cells due to electrostatic repulsion from the cell membrane and is easily degraded by RNases. By encapsulating RNA within LNP, RNA can effectively traverse the cell membrane and be released into the cytoplasm, enabling efficient delivery.

MCE In vivo OptiLNP RNA Transfection Reagent (Local Effect) is a ready-to-use transfection reagent based on LNP technology. It utilizes the unique transmembrane transport and escape mechanism of LNP to achieve efficient delivery of RNA. It is suitable for In vivo transfection of mRNA and short-chain RNA in experimental animals, including but not limited to intramuscular injection, intravenous injection, subcutaneous injection, and intraperitoneal injection. The RNA functions locally.

Features of MCE In vivo OptiLNP RNA Transfection Reagent (Local Effect)

1. Lipid Nanoparticle (LNP) Technology: Utilizes LNP carriers to effectively protect RNA from degradation and immune system clearance during the delivery process.

2. Efficient Intracellular Delivery: The unique transmembrane transport and escape mechanisms of LNPs achieve an encapsulation rate of over 80% for small sample amounts, enabling efficient transfection of RNA.

3. Wide Applicability: Suitable for in vivo transfection of mRNA and short-chain RNA in experimental animals.

4. User-Friendly Operation: No encapsulation equipment is required; RNA-LNP encapsulation and transfection can be completed in a single mixing step.

5. Biocompatibility: Extremely low toxicity in animals with mild effects.

6. Safety: The formulation uses biodegradable lipid materials to ensure safety.

Transfection Reagent, Dilution Buffer: 4℃, 1 year. Do not freeze.

Fluc mRNA Positive Control (Lyophilized): -20℃, 1 year.

1. Prepare RNA Pre-mix Solution: Dilute the mRNA stock solution with the dilution buffer.

2. Prepare RNA-LNP Complex: Add the transfection reagent to the RNA pre-mixed solution and mix thoroughly by pipetting up and down at least 10 times.

3. Injection: Inject the RNA-LNP complex into the animal.

1. The actual dosage and injection volume of RNA should be determined based on a comprehensive consideration of the animal model, administration route, and target organ.

2. It is recommended that the stock concentration of mRNA be ≥ 1 μg/μL, and the concentration of mRNA in the RNA-LNP complex be ≤ 0.2 μg/μL. For siRNA, the stock concentration should be ≥ 2 μg/μL, and the concentration of siRNA in the RNA-LNP complex should be ≤ 1 μg/μL.

The delivery and transfection efficiency can be evaluated using conventional detection methods, including:

Fluorescence imaging system: Detecting fluorescence signals to assess delivery efficiency.

RT-qPCR: Quantifying target gene expression levels.

Western Blot: Detecting target protein expression.

Immunofluorescence: Localizing target protein expression.

Additionally, tissue sampling and distribution analysis, as well as animal phenotype and physiological analysis, should be performed to evaluate the transfection delivery effectiveness.

1. This product has been filtered using a 0.22 μm membrane.

2. The Fluc mRNA Positive Control is provided as a lyophilized powder. Please centrifuge before opening the cap. Use nuclease-free water to dissolve and prepare a positive control stock solution at 1 μg/μL. The stock solution can be stored at -80°C for 6 months, avoiding repeated freeze-thaw cycles.

3. For optimal transfection efficiency, it is recommended to dissolve the RNA stock in nuclease-free sterile water. Other solvents may affect transfection efficiency. It is recommended to use commercially available RNA; for self-prepared RNA, purification and desalting are necessary to prevent RNA precipitation.

4. It is recommended to prepare RNA pre-mixes using the dilution buffer provided with the kit to dilute the RNA stock solution.

5. Common short-chain RNAs (e.g., siRNA) typically weigh between 13 - 15 μg per 1 nmol without special modifications. The exact weight can be calculated based on the specific nucleic acid sequence and modifications.

6. RNA-LNP complexes can be stably stored at room temperature for up to 8 h. Do not store RNA-LNP complexes under freezing conditions.

7. If dilution of the RNA-LNP complex is required, it is recommended to use the dilution buffer provided with the kit for dilution.

8. Under suitable conditions, repeated transfection administration can also be performed.

9. To avoid RNAse contamination, RNA-free enzymes and non-pyrogenic materials should be used throughout the experiment.

10. This product is for R&D use only, not for drug, household, or other uses.

11. For your safety and health, please wear a lab coat and disposable gloves to operate.