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  3. Fluorescent Dye
  4. Janelia Fluor® 669, SE

Janelia Fluor® 669, SE  (Synonyms: JF669, SE; JF669, NHS)

Cat. No.: HY-131030 Purity: ≥94.0%
COA Handling Instructions

Molarity Calculator

Janelia Fluor 669, SE (JF669, SE), a red fluorescent dye, can be directly reacted with the available thiol-containing HaloTag ligand under mild conditions (DIEA, DMF) to afford a JF669HaloTag ligand in a single step (Ex = 669 nm; Em = 682 nm). Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

For research use only. We do not sell to patients.

Janelia Fluor® 669, SE Chemical Structure

Janelia Fluor® 669, SE Chemical Structure

CAS No. : 2127150-20-1

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Based on 1 publication(s) in Google Scholar

Janelia Fluor® 669, SE Related Antibodies

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Description

Janelia Fluor 669, SE (JF669, SE), a red fluorescent dye, can be directly reacted with the available thiol-containing HaloTag ligand under mild conditions (DIEA, DMF) to afford a JF669HaloTag ligand in a single step (Ex = 669 nm; Em = 682 nm)[1]. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

In Vitro

JF669 undergoes rapid conversion to thioether product (<5 min to completion) in organic solvents, where the dye predominantly adopts the closed form[1].
JF669 displays comparatively excellent photostability, retaining 97% fluorescence after the same number of cycles[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Janelia Fluor® 669, SE Related Antibodies
Molecular Weight

693.76

Formula

C34H30F3N3O6SSi
CAS No.
Appearance

Solid

Color

Light green to green

Emission (Em)

669
Excitation (Ex)

640
SMILES

C[Si](C)(C1=C/2)C3=CC(N4CCC4)=CC=C3C(C5=C(C(SCC(ON6C(CCC6=O)=O)=O)=C(C(F)=C5C([O-])=O)F)F)=C1C=CC2=[N+]7CCC/7
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Purity & Documentation

Purity: ≥94.0%

COA (258 KB)

Handling Instructions (2659 KB)
References
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Janelia Fluor® 669, SE Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Janelia Fluor® 669, SE2127150-20-1JF669, SE JF669, NHSFluorescent DyeInhibitorinhibitorinhibit

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