Janelia Fluor® 669, SE
Janelia Fluor 669, SE (JF669, SE), a red fluorescent dye, can be directly reacted with the available thiol-containing HaloTag ligand under mild conditions (DIEA, DMF) to afford a JF669HaloTag ligand in a single step (Ex = 669 nm; Em = 682 nm). Janelia Fluor fluorescent dyes can be chemically conjugated to the specific HaloTag substrate (a chloroalkane ligand) to form a fluorescent ligand, rather than binding directly to the HaloTag protein itself. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.
For research use only. We do not sell to patients.
- CAS No.: 2127150-20-1
- Formula: C34H30F3N3O6SSi
- Molecular Weight:693.76
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Biological Activity
Janelia Fluor fluorescent dyes do not inherently possess HaloTag-binding activity; however, reactive groups such as NHS esters serve as reaction sites for covalent coupling-via chemical synthesis-to an amino-functionalized HaloTag chloroalkane ligand scaffold, thereby yielding a fluorescent HaloTag ligand.
JF669 undergoes rapid conversion to thioether product (<5 min to completion) in organic solvents, where the dye predominantly adopts the closed form[1].
JF669 displays comparatively excellent photostability, retaining 97% fluorescence after the same number of cycles[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
669
640
Chemical Information
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CAS No. 2127150-20-1
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Appearance Solid
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Molecular Weight 693.76
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Formula C34H30F3N3O6SSi
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Color Off-white to light yellow
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SMILES
C[Si](C)(C1=C/2)C3=CC(N4CCC4)=CC=C3C(C5=C(C(SCC(ON6C(CCC6=O)=O)=O)=C(C(F)=C5C([O-])=O)F)F)=C1C=CC2=[N+]7CCC/7
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Synonyms
JF669, SE; JF669, NHS
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)