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  4. ​JOE

JOE is a xanthene fluorophore (i.e., 4′,5′-dichloro-2′,7′-dimethoxy-5 (6)-carboxyfluorescein; 2',7'-dimethoxy-4',5'-dichloro-6-carboxyfluorescein) with an absorption wavelength of approximately 525 nm and an emission wavelength of approximately 550 nm. The fluorescence quantum yield of JOE correlates with the rigidity of the linker arm and the distance to dG nucleoside. JOE is commonly used as a fluorescent label for oligonucleotides and molecular beacon probes, and also serves as the acceptor fluorophore in fluorescence energy transfer primers for DNA sequencing.

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​JOE

​JOE 構造式

CAS 番号 : 82855-40-1

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 28 在庫あり
Solution
10 mM * 1 mL in DMSO USD 28 在庫あり
Solid
5 mg $25 在庫あり
10 mg $36 在庫あり
25 mg $75 在庫あり
50 mg $113 在庫あり
100 mg $180 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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​JOE 関連抗体

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製品説明

JOE is a xanthene fluorophore (i.e., 4′,5′-dichloro-2′,7′-dimethoxy-5 (6)-carboxyfluorescein; 2',7'-dimethoxy-4',5'-dichloro-6-carboxyfluorescein) with an absorption wavelength of approximately 525 nm and an emission wavelength of approximately 550 nm. The fluorescence quantum yield of JOE correlates with the rigidity of the linker arm and the distance to dG nucleoside. JOE is commonly used as a fluorescent label for oligonucleotides and molecular beacon probes, and also serves as the acceptor fluorophore in fluorescence energy transfer primers for DNA sequencing[1][2][3].

体外実験

The fluorescence quantum yield of JOE (in 1× TBE/7 M urea solution) relative to fluorescein is 0.58, and its emission intensity is lower than that of the F10J ET primer when excited at 488 nm or 514 nm[2].
JOE-labeled molecular beacons exhibit higher fluorescence signals in the hairpin state than in the double-stranded state formed with complementary target ODN, and the signal levels vary with different probe types and linkers[3].
When the JOE-labeled probe forms a hairpin state and a double-stranded state with the complementary target ODN, it exhibits distinct fluorescence melting curve characteristics, thus enabling the differentiation of these two conformations[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

​JOE 関連抗体
分子量

505.26

分子式

C23H14Cl2O9
CAS 番号
Appearance

Solid

Color

Brown to reddish brown

SMILES

O=C(C1=CC(C2(C3=C(OC4=C2C=C(OC)C(O)=C4Cl)C(Cl)=C(O)C(OC)=C3)O5)=C(C=C1)C5=O)O
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : 50 mg/mL (98.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 5 mg/mL (9.90 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9792 mL 9.8959 mL 19.7918 mL
5 mM 0.3958 mL 1.9792 mL 3.9584 mL
10 mM 0.1979 mL 0.9896 mL 1.9792 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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mg/kg

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(per animal)

g

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(per animal)

μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
純度とドキュメンテーション
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.9792 mL 9.8959 mL 19.7918 mL 49.4795 mL
5 mM 0.3958 mL 1.9792 mL 3.9584 mL 9.8959 mL
DMSO 10 mM 0.1979 mL 0.9896 mL 1.9792 mL 4.9479 mL
15 mM 0.1319 mL 0.6597 mL 1.3195 mL 3.2986 mL
20 mM 0.0990 mL 0.4948 mL 0.9896 mL 2.4740 mL
25 mM 0.0792 mL 0.3958 mL 0.7917 mL 1.9792 mL
30 mM 0.0660 mL 0.3299 mL 0.6597 mL 1.6493 mL
40 mM 0.0495 mL 0.2474 mL 0.4948 mL 1.2370 mL
50 mM 0.0396 mL 0.1979 mL 0.3958 mL 0.9896 mL
60 mM 0.0330 mL 0.1649 mL 0.3299 mL 0.8247 mL
80 mM 0.0247 mL 0.1237 mL 0.2474 mL 0.6185 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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​JOE Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

​JOE82855-40-1DNA StainFluorescent Dyexanthene fluorophorefluorescence energy transfer primersDNA sequencingM13mp18 templatedG nucleosidesphosphoramidite reagentsoligonucleotidesmolecular beacon probesfluorescent dyeisovanillinInhibitorinhibitorinhibit

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製品名:
​JOE
製品番号:
HY-D1090
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