Lotus tetragonolobus lectin  (Synonyms: LTL)

Lotus tetragonolobus lectin (LTL) is a plant lectin that specifically recognizes and binds to α-L-fucopyranosyl residues, a sugar structure serving as the key terminal glycosyl group of human blood type O antigen (H antigen). Lotus tetragonolobus lectin exerts macrophage migration inhibitory activity in monomeric form. Lotus tetragonolobus lectin labels and identifies renal proximal tubular epithelial cells to evaluate histopathological changes of sepsis-induced acute kidney injury. Lotus tetragonolobus lectin is applicable to studies in glycobiology, immunology and renal pathology^[1][2].

Purified Lotus tetragonolobus lectin (1.9 μg/mL) agglutinates a 0.3% suspension of type O (d) human erythrocytes at the minimum concentration of 1.9 μg/mL^[1].
Purified Lotus tetragonolobus lectin consists of three fucose-specific isolectins, which is confirmed by the presence of three precipitin arcs in immunoelectrophoresis against rabbit antiserum raised against crude LTL^[1].
Purified Lotus tetragonolobus lectin (0.5-500 ng/mL; 16 hrs) inhibits the migration of guinea pig peritoneal macrophages in a bell-shaped dose-dependent manner; its effect is mediated by surface receptors containing α-L-fucopyranosyl groups^[1].
Purified Lotus tetragonolobus lectin (0.01-100 μg/mL; 0-120 h) exhibits no mitogenic activity toward lymphocytes isolated from normal guinea pig lymph nodes at concentrations ranging from 0.01 to 100 μg/mL and with incubation periods up to 120 h^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Lotus tetragonolobus lectin (local application; for immunofluorescence staining of fixed kidney tissue sections) acts as a specific fluorescent marker for renal proximal tubules, allowing quantitative assessment of sepsis-induced acute kidney injury via counting of LCN2-positive injured tubular cells in mouse kidney tissue^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Solid

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[Lotus tetragonolobus lectin]

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• [1]. Fahlbusch B, et al. Interaction of Lotus-tetragonolobus Lectin (LTL) and an MIF-like factor with guinea-pig macrophages. I. Effects on macrophage migration inhibition and receptor-binding studies. Immunobiology. 1982;162(3):251-264.  [Content Brief]

  [2]. Deng Z, et al. Melatonin attenuates sepsis-induced acute kidney injury by promoting mitophagy through SIRT3-mediated TFAM deacetylation. Autophagy. 2024;20(1):151-165.  [Content Brief]