2. Academic Validation
  3. Preparation of highly purified momordin II without ribonuclease activity

Preparation of highly purified momordin II without ribonuclease activity

  • Life Sci. 1999;65(14):1485-91. doi: 10.1016/s0024-3205(99)00389-6.
P Valbonesi 1,  L Barbieri,  A Bolognesi,  E Bonora,  L Polito,  F Stirpe
Affiliations

Affiliation

  • 1 Dipartimento di Patologia Sperimentale, Università di Bologna, Italy.
PMID: 10530800 DOI: 10.1016/s0024-3205(99)00389-6
Abstract

Momordin II, a ribosome-inactivating protein from Momordica charantia seeds, was purified by a procedure involving a series of chromatographies on S-Sepharose, Sephadex G-50, CM-Sepharose, and Red Sepharose columns. Highly purified momordin II inhibited cell-free protein synthesis, released adenine from rat liver ribosomes and from DNA, and had no RNase activity.

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