Nucleophosmin/B23 is a target of CDK2/cyclin E in centrosome duplication

Cell. 2000 Sep 29;103(1):127-40. doi: 10.1016/s0092-8674(00)00093-3.

M Okuda ¹, H F Horn, P Tarapore, Y Tokuyama, A G Smulian, P K Chan, E S Knudsen, I A Hofmann, J D Snyder, K E Bove, K Fukasawa

Affiliations

Affiliation

1 Department of Cell Biology, Neurobiology, and Anatomy, University of Cincinnati College of Medicine, Ohio 45267, USA.

PMID: 11051553 DOI: 10.1016/s0092-8674(00)00093-3

Abstract

In animal cells, duplication of centrosomes and DNA is coordinated. Since CDK2/cyclin E triggers initiation of both events, activation of CDK2/cyclin E is thought to link these two events. We identified nucleophosmin (NPM/B23) as a substrate of CDK2/cyclin E in centrosome duplication. NPM/B23 associates specifically with unduplicated centrosomes, and NPM/B23 dissociates from centrosomes by CDK2/cyclin E-mediated phosphorylation. An anti-NPM/B23 antibody, which blocks this phosphorylation, suppresses the initiation of centrosome duplication in vivo. Moreover, expression of a nonphosphorylatable mutant NPM/ B23 in cells effectively blocks centrosome duplication. Thus, NPM/B23 is a target of CDK2/cyclin E in the initiation of centrosome duplication.

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