2. Academic Validation
  3. Two-step enzymatic synthesis of UDP-N-acetylgalactosamine

Two-step enzymatic synthesis of UDP-N-acetylgalactosamine

  • Bioorg Med Chem Lett. 2005 Dec 15;15(24):5459-62. doi: 10.1016/j.bmcl.2005.08.088.
Vanessa Bourgeaux 1,  Friedrich Piller,  Véronique Piller
Affiliations

Affiliation

  • 1 Centre de Biophysique Moléculaire CNRS UPR4301 affiliée à l'Université d'Orléans et à l'INSERM, rue Charles Sadron, F-45071 Orléans Cedex 02, France.
PMID: 16203137 DOI: 10.1016/j.bmcl.2005.08.088
Abstract

UDP-GalNAc has been synthesised with high yield from GalNAc, UTP and ATP using recombinant human GalNAc kinase GK2 and UDP-GalNAc pyrophosphorylase AGX1. Both enzymes have been prepared in one step from 1L cultures of transformed Escherichia coli and the UDP-GalNAc produced has been purified by a simple procedure. The method described is a rapid and efficient means to produce UDP-GalNAc as well as analogues like UDP-N-azidoacetylgalactosamine (UDP-GalNAz).

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