2. Academic Validation
  3. Simultaneous determination of three aconitum alkaloids in urine by LC-MS-MS

Simultaneous determination of three aconitum alkaloids in urine by LC-MS-MS

  • J Pharm Biomed Anal. 2007 Sep 21;45(1):145-148. doi: 10.1016/j.jpba.2007.04.016.
ZhaohongWang 1,  Zhiping Wang 2,  Jiao Wen 2,  Yi He 2
Affiliations

Affiliations

  • 1 Forensic Science Institute of Public Security Ministry, Beijing 100038, PR China. Electronic address: [email protected].
  • 2 Forensic Science Institute of Public Security Ministry, Beijing 100038, PR China.
PMID: 17555909 DOI: 10.1016/j.jpba.2007.04.016
Abstract

A sensitive method has been developed for the identification and quantification of the toxic Alkaloids yunaconitine, crassicauline A, and foresaconitine in urine specimens. After solid-phase extraction using Oasis MCX cartridges, the extracts were analyzed by LC-MS-MS. The limit of detection is 0.03 ng/mL urine for yunaconitine and 0.05 ng/mL urine for crassicauline A and foresaconitine; the limit of quantitation is 0.15 ng/mL urine for yunaconitine and 0.20 ng/mL urine for crassicauline A and foresaconitine. The method was employed in the analysis of the urine of a 55-year-old male who died after ingestion of herbal medicine powder made from the roots of aconite. Yunaconitine, crassicauline A and foresaconitine were identified in the urine. Crassicauline A and foresaconitine were thus identified in a biological specimen for the first time.

Figures
Products