2. Academic Validation
  3. Site specific covalent alkylation of DNA by antitumor antibiotics, duocarmycin A and kapurimycin A3

Site specific covalent alkylation of DNA by antitumor antibiotics, duocarmycin A and kapurimycin A3

  • Nucleic Acids Symp Ser. 1991:(25):75-6.
H Sugiyama 1,  C K Lam,  M Hosoda,  I Saito
Affiliations

Affiliation

  • 1 Department of Synthetic Chemistry, Faculty of Engineering, Kyoto University, Japan.
PMID: 1842103
Abstract

DNA alkylation by two different antitumor Antibiotics, duocarmycin A and kapurimycin A3 was investigated by using of synthetic oligonucleotides. It was found that duocarmycin A reacted with N3 of adenine6 in d(CGTATACG)2 to provide a stable adduct. Kapurimycin A3 was found to react with d(CGCG)2 at N7 of guanine4 to yield a covalent adduct. Under heating conditions both adducts decompose to give an abasic-containing oligomer and a drug-base adduct.

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