2. Academic Validation
  3. Identification of a novel protein binding to hepatitis C virus core protein

Identification of a novel protein binding to hepatitis C virus core protein

  • J Gastroenterol Hepatol. 2009 Jul;24(7):1300-4. doi: 10.1111/j.1440-1746.2009.05846.x.
Yun-Ru Chen 1,  Tian-Yan Chen,  Shu-Lin Zhang,  Shu-Mei Lin,  Ying-Ren Zhao,  Feng Ye,  Xi Zhang,  Lei Shi,  Shuang-Suo Dang,  Min Liu
Affiliations

Affiliation

  • 1 The First Affiliated Hospital, Medical School, Xi'an Jiaotong University, Xi'an, Shaanxi Province, China.
PMID: 19486448 DOI: 10.1111/j.1440-1746.2009.05846.x
Abstract

Background: Hepatitis C virus (HCV) core protein is a multi-functional viral protein that interacts with several target proteins of both viral and cellular origin.

Aim and methods: To gain insight into the mechanism of action of HCV core protein, we used a yeast two-hybrid system to identify the core protein-interacting cellular targets.

Results: A cDNA clone encoding an aspartoacylase was obtained, termed aspartoacylase 3 (ACY3). Interaction between ACY3 and HCV core protein was verified using a co-immunoprecipitation assay in vitro, and a mammalian two-hybrid system in vivo. Fluorescence microscopy showed green fluorescence protein-fused ACY3 localized in the cytoplasm.

Conclusion: Our data suggest that ACY3 is an HCV core binding protein, which may play a role in the development of HCV-associated diseases.

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