2. Academic Validation
  3. Cyanine fluorophore derivatives with enhanced photostability

Cyanine fluorophore derivatives with enhanced photostability

  • Nat Methods. 2011 Nov 13;9(1):68-71. doi: 10.1038/nmeth.1774.
Roger B Altman 1,  Daniel S Terry,  Zhou Zhou,  Qinsi Zheng,  Peter Geggier,  Rachel A Kolster,  Yongfang Zhao,  Jonathan A Javitch,  J David Warren,  Scott C Blanchard
Affiliations

Affiliation

  • 1 Department of Physiology and Biophysics, Weill Medical College of Cornell University, New York, New York, USA.
PMID: 22081126 DOI: 10.1038/nmeth.1774
Abstract

Fluorescence applications requiring high photostability often depend on the use of solution additives to enhance fluorophore performance. Here we demonstrate that the direct or proximal conjugation of cyclooctatetraene (COT), 4-nitrobenzyl alcohol (NBA) or Trolox to the cyanine fluorophore Cy5 dramatically enhanced fluorophore photostability without otherwise affecting its native spectral characteristics. Such conjugation is a powerful means of improving the robustness of fluorescence-based applications demanding long-lived, nonblinking fluorescence emission.

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