2. Academic Validation
  3. Pregnane alkaloids from Sarcococca hookeriana var. digyna

Pregnane alkaloids from Sarcococca hookeriana var. digyna

  • Fitoterapia. 2013 Sep:89:143-8. doi: 10.1016/j.fitote.2013.04.010.
Pu-zhao Zhang 1,  Fei Wang,  Li-juan Yang,  Guo-lin Zhang
Affiliations

Affiliation

  • 1 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, PR China.
PMID: 23651560 DOI: 10.1016/j.fitote.2013.04.010
Abstract

Fourteen pregnane-type steroidal Alkaloids were isolated from the ethanolic extracts of whole Sarcococca hookeriana var. digyna Plants. Their structures were elucidated on the basis of spectral data. Three of them were identified as new steroidal alkaloids: (S)-20-(N,N-dimethylamino)-16α,17α-epoxy-3β-methoxy-pregn-5-ene (1), (20S)-20-(N,N-dimethylamino)-3β-tigloylamino-5α-pregn-11β-ol (2), and (20S)-2α,4β-bis(acetoxy)-20-(N,N-dimethylamino)-3β-tigloylamino-5α-pregnane (3). Some of the isolated compounds showed estrogen biosynthesis-promoting effects in human ovarian granulosa-like KGN cells. The EC50 values for the most effective compounds, vagnine B (6) and funtumafrine C (12), were 71 μM and 67 μM, respectively.

Keywords

Akt; CREB; ERK; Estrogen production; KGN cell; MAPK; PI3K; PKA; Pregnane; Sarcococca hookeriana var. digyna; Steroidal alkaloid; cAMP; cAMP response element binding protein; cyclic adenosine monophosphate; extracellular signal-regulated kinase; mTOR; mammalian target of rapamycin; mitogen-activated protein kinase; phosphatidylinositol-3 kinase; protein kinase A; protein kinase B.

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