1. Academic Validation
  2. Mast cell tryptase induces eosinophil recruitment in the pleural cavity of mice via proteinase-activated receptor 2

Mast cell tryptase induces eosinophil recruitment in the pleural cavity of mice via proteinase-activated receptor 2

  • Inflammation. 2013 Dec;36(6):1260-7. doi: 10.1007/s10753-013-9664-5.
Natália A Matos 1 Josiane F Silva Tamires C Matsui Karine A Damasceno Igor D G Duarte Virginia S Lemos Geovanni D Cassali André Klein
Affiliations

Affiliation

  • 1 Department of Pharmacology, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Abstract

Proteinase-activated receptor (PAR) 2 has been implicated in eosinophil migration. Mast cell (MC) tryptase has been similarly implicated in allergic diseases through the activation of PAR-2, but the role of this receptor in MC tryptase-induced inflammation is not well elucidated. This study aims to investigate the ability of MC tryptase or PAR-2 activating peptide (SLIGRL-NH2) to induce eosinophil recruitment to the pleural cavity of mice. Mast cell tryptase-injected mice were pretreated with PAR-2 antagonist ENMD-1068. Mice injected with SLIGRL-NH2 were pretreated with mast cell tryptase inhibitor APC 366, and eosinophil migration into the pleural cavity and PAR-2 expression was analyzed after 24 or 48 h. SLIGRL-NH2-induced eosinophil recruitment was inhibited by APC 366, and MC tryptase-induced eosinophil recruitment was abolished by ENMD-1068. MC tryptase induced PAR-2 expression on pleural eosinophils. Our results demonstrate a key role for PAR-2 in mediating eosinophil recruitment in MC tryptase-induced pleurisy in mice. The ability of MC tryptase to inducing PAR-2 expression on eosinophils corroborates the relevance of MC tryptase and PAR-2 on modulating eosinophil migration.

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Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-105999B
    ≥99.0%, Mast Cell Tryptase Inhibitor