The antioxidant activity of daidzein metabolites, O‑desmethylangolensin and equol, in HepG2 cells

Daidzein and its glycoside form daidzin, are known to have potential health benefits and are metabolized to O‑desmethylangolensin (O‑DMA) and equol following consumption. In the current study, the antioxidant activity and cytotoxicity of O‑DMA, equol, daidzein and daidzin was investigated and their effects on HepG2 human hepatocelluar carcinoma cells were compared. For cytotoxicity assays, lactose dehydrogenase (LDH) release and 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide‑based cell viability, cells were exposed to various concentrations of each compound (5‑200 µM) for 24, 48 or 72 h. O‑DMA and equol did not affect LDH release, but higher concentrations (<75 µM) showed inhibition of cell growth. By contrast, daidzein and daidzin (200 µM) increased LDH release and cell growth. All compounds stimulated catalase and total superoxide dismutase (SOD) (CuZn‑ and Mn‑SOD) activity, and mRNA and protein expression. This phenomenon was most pronounced for O‑DMA and equol, as their effects were similar. These data suggested that O‑DMA and equol possess greater antioxidant properties compared with daidzein and may, thus, be beneficial for human health.