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  3. A new strategy for recovery of two peptides without Glu employing glutamate-specific endopeptidase from Bacillus licheniformis

A new strategy for recovery of two peptides without Glu employing glutamate-specific endopeptidase from Bacillus licheniformis

  • Enzyme Microb Technol. 2014 Jan 10:54:25-31. doi: 10.1016/j.enzmictec.2013.09.013.
Wei Ye 1,  Yi Ma,  Haiying Wang,  Xiaochun Luo,  Weimin Zhang,  Jufang Wang,  Xiaoning Wang
Affiliations

Affiliation

  • 1 School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China; State Key Laboratory of Applied Microbiology, South China (The Ministry-Province Joint Development), Guangdong Institute of Microbiology, GuangZhou 510070, PR China.
PMID: 24267564 DOI: 10.1016/j.enzmictec.2013.09.013
Abstract

The difficulty in the purification of bioactive peptide limited its application in food, drug and cosmetic industry. Here we report a new strategy for the recovery of two Peptides employing glutamate-specific endopeptidase from Bacillus licheniformis (GSE-BL), which shows strong specificity for Glu residue. Human glucagon and human beta-defensin-2 (HBD-2) were Peptides without Glu residue, and Glu residue was introduced between affinity tag and target peptide as recognition site of GSE-BL. Tagless human glucagon with the same HPLC retention time as native human glucagon and mature HBD-2 with Antibacterial activity and cytotoxicity were obtained after GSE-BL treatment. This strategy has great potential in the recovery of bioactive peptide without Glu residue, thus facilitating large scale preparation of peptide and widening the application of bioactive peptide.

Keywords

GSE-BL; HBD; His-Tag; Human beta defensin-2; Human glucagon; glutamate-specific endopeptidase from Bacillus licheniformis; human beta defensin.

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