Z-Phe-Leu-Glu-pNA
Based on 1 Customer Validation
Z-Phe-Leu-Glu-pNA is a chromogenic substrate for glutamyl endopeptidases. Z-Phe-Leu-Glu-pNA has been used as a synthetic substrate in the study of proteolytic enzymes, including trypsin treatment, subtilisin and chymotrypsin. Z-Phe-Leu-Glu-pNA plays an important role in hormone disorders such as prostate cancer and breast cancer.
For research use only. We do not sell to patients.
- Purity: 99.48%
- CAS No.: 104634-10-8
- Formula: C34H39N5O9
- Molecular Weight:661.70
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Biological Activity
others
Chemical Information
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CAS No. 104634-10-8
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Appearance Solid
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Molecular Weight 661.70
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Formula C34H39N5O9
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Color White to off-white
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Sequence
Z-Phe-Leu-{Glu-pNA}
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Sequence Shortening
Z-FL-{Glu-pNA}
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : ≥ 50 mg/mL (75.56 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Wei Ye, et al. Characterization of the glutamate-specific endopeptidase from Bacillus licheniformis expressed in Escherichia coli. J Biotechnol. 2013 Oct 10;168(1):40-5. [Content Brief]
[2]. Wei Ye, et al. A new strategy for recovery of two peptides without Glu employing glutamate-specific endopeptidase from Bacillus licheniformis. Enzyme Microb Technol. 2014 Jan 10:54:25-31. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5113 mL | 7.5563 mL | 15.1126 mL | 37.7815 mL |
| 5 mM | 0.3023 mL | 1.5113 mL | 3.0225 mL | 7.5563 mL | |
| 10 mM | 0.1511 mL | 0.7556 mL | 1.5113 mL | 3.7781 mL | |
| 15 mM | 0.1008 mL | 0.5038 mL | 1.0075 mL | 2.5188 mL | |
| 20 mM | 0.0756 mL | 0.3778 mL | 0.7556 mL | 1.8891 mL | |
| 25 mM | 0.0605 mL | 0.3023 mL | 0.6045 mL | 1.5113 mL | |
| 30 mM | 0.0504 mL | 0.2519 mL | 0.5038 mL | 1.2594 mL | |
| 40 mM | 0.0378 mL | 0.1889 mL | 0.3778 mL | 0.9445 mL | |
| 50 mM | 0.0302 mL | 0.1511 mL | 0.3023 mL | 0.7556 mL | |
| 60 mM | 0.0252 mL | 0.1259 mL | 0.2519 mL | 0.6297 mL |