2. Academic Validation
  3. Five withanolides from the leaves of Datura metel L. and their inhibitory effects on nitric oxide production

Five withanolides from the leaves of Datura metel L. and their inhibitory effects on nitric oxide production

  • Molecules. 2014 Apr 11;19(4):4548-59. doi: 10.3390/molecules19044548.
Bing-You Yang 1,  Rui Guo 1,  Ting Li 1,  Yan Liu 1,  Chang-Fu Wang 1,  Zun-Peng Shu 1,  Zhi-Bin Wang 1,  Jing Zhang 1,  Yong-Gang Xia 1,  Hai Jiang 1,  Qiu-Hong Wang 2,  Hai-Xue Kuang 3
Affiliations

Affiliations

  • 1 Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China.
  • 2 Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China. [email protected].
  • 3 Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China. [email protected].
PMID: 24731984 DOI: 10.3390/molecules19044548
Abstract

Four new withanolides named dmetelins A-D (compounds 1-4), along with the known compound 7α,27-dihydroxy-1-oxo-witha-2,5,24-trienolide (5) were isolated from the leaves of Datura metel L. (Solanaceae). Their structures were elucidated on the basis of detailed analysis of 1D and 2D NMR and mass spectrometry data. All the compounds were evaluated for their inhibitory effects on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW264.7 cells. Compounds 1, 4 and 5 showed significant inhibitory activities, and compounds 2 and 3 showed moderate inhibitory activities with IC50 values of 17.8, 11.6, 14.9, 33.3 and 28.6 μM, respectively.

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