2. Academic Validation
  3. Anti-inflammatory effects of glaucocalyxin B in microglia cells

Anti-inflammatory effects of glaucocalyxin B in microglia cells

  • J Pharmacol Sci. 2015 May;128(1):35-46. doi: 10.1016/j.jphs.2015.04.005.
Ping Gan 1,  Li Zhang 1,  Yanke Chen 1,  Yu Zhang 1,  Fali Zhang 1,  Xiang Zhou 1,  Xiaohu Zhang 2,  Bo Gao 1,  Xuechu Zhen 1,  Jian Zhang 3,  Long Tai Zheng 4
Affiliations

Affiliations

  • 1 Jiangsu Key Laboratory of Translational Research and Therapy for Neuropsychiatric Disorders & Department of Pharmacology, College of Pharmaceutical Sciences and the Collaborative Innovation Center for Brain Science, Soochow University, Suzhou 215123, PR China.
  • 2 Department of Medical Chemistry, College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, PR China.
  • 3 Department of Natural Medical Chemistry, College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, PR China. Electronic address: [email protected].
  • 4 Jiangsu Key Laboratory of Translational Research and Therapy for Neuropsychiatric Disorders & Department of Pharmacology, College of Pharmaceutical Sciences and the Collaborative Innovation Center for Brain Science, Soochow University, Suzhou 215123, PR China. Electronic address: [email protected].
PMID: 26003084 DOI: 10.1016/j.jphs.2015.04.005
Abstract

Over-activated microglia is involved in various kinds of neurodegenerative process including Parkinson, Alzheimer and HIV dementia. Suppression of microglial over activation has emerged as a novel strategy for treatment of neuroinflammation-based neurodegeneration. In the current study, anti-inflammatory and neuroprotective effects of the ent-kauranoid Diterpenoids, which were isolated from the aerial parts of Rabdosia japonica (Burm. f.) var. glaucocalyx (Maxim.) Hara, were investigated in cultured microglia cells. Glaucocalyxin B (GLB), one of five ent-kauranoid Diterpenoids, significantly decreased the generation of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, cyclooxygenase (COX)-2 and inducible nitric oxide synthase (iNOS) in the lipopolysaccharide (LPS)-activated microglia cells. In addition, GLB inhibited activation of nuclear factor-κB (NF-κB), p38 mitogen-activated protein kinase (MAPK) and generation of Reactive Oxygen Species (ROS) in LPS-activated microglia cells. Furthermore, GLB strongly induced the expression of heme oxygenase (HO)-1 in BV-2 microglia cells. Finally, GLB exhibited neuroprotective effect by preventing over-activated microglia induced neurotoxicity in a microglia/neuron co-culture model. Taken together, the present study demonstrated that the GLB possesses anti-nueroinflammatory activity, and might serve as a potential therapeutic agent for treating neuroinflammatory diseases.

Keywords

Glaucocalyxin B; Microglia; NF-κB; Neuroprotection; Reactive oxygen species.

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