1. Academic Validation
  2. Impact of two different colistin dosing strategies on healthy piglet fecal microbiota

Impact of two different colistin dosing strategies on healthy piglet fecal microbiota

  • Res Vet Sci. 2016 Aug;107:152-160. doi: 10.1016/j.rvsc.2016.06.003.
M A Fleury 1 E Jouy 2 F Eono 2 R Cariolet 2 W Couet 3 P Gobin 4 O Le Goff 5 S Blanquet-Diot 5 M Alric 5 I Kempf 6
Affiliations

Affiliations

  • 1 ANSES, Laboratoire de Ploufragan-Plouzané, Ploufragan, France; Université Bretagne, Loire, France; Clermont Université, Université d'Auvergne, Centre de Recherche en Nutrition Humaine Auvergne, EA 4678 CIDAM, Conception, Ingénierie et Développement de l'Aliment et du Médicament, Clermont-Ferrand, France.
  • 2 ANSES, Laboratoire de Ploufragan-Plouzané, Ploufragan, France; Université Bretagne, Loire, France.
  • 3 Inserm U1070, Pôle Biologie Santé, Poitiers, France; Université de Poitiers, UFR Médecine-Pharmacie, Poitiers, France; CHU Poitiers, Service de Toxicologie-Pharmacocinétique, Poitiers, France.
  • 4 CHU Poitiers, Service de Toxicologie-Pharmacocinétique, Poitiers, France.
  • 5 Clermont Université, Université d'Auvergne, Centre de Recherche en Nutrition Humaine Auvergne, EA 4678 CIDAM, Conception, Ingénierie et Développement de l'Aliment et du Médicament, Clermont-Ferrand, France.
  • 6 ANSES, Laboratoire de Ploufragan-Plouzané, Ploufragan, France; Université Bretagne, Loire, France. Electronic address: [email protected].
Abstract

Colistin is often used in piglets but underdosing and overdosing are frequent. The impact of such administrations on fecal microbiota was studied. Piglets were given either underdoses of colistin by oral gavage for five days or overdoses by in-feed medication for 14days. The composition of fecal microbiota was studied by quantitative PCR, 16S rRNA sequencing, culture of Enterobacteriaceae, and quantification of short-chain fatty acids (SCFAs). The mean colistin concentrations during the treatment for underdosed and overdosed groups were 14.4μg/g and 64.9μg/g of feces respectively. Whatever the piglet and the sampling day, the two main phyla were Firmicutes and Bacteroidetes, The main families were Lactobacillaceae, Clostridiales, Lachnospiraceae and Ruminococcaceae. The main perturbation was the significant but transitory decrease in the Escherichia coli population during treatment, yet all the E. coli isolates were susceptible to colistin. Moreover, colistin did not affect the production of SCFAs. These results show that under- or overdoses of colistin do not result in any major disturbance of piglet fecal microbiota and rarely select for chromosomal resistance in the dominant E. coli population.

Keywords

Colistin; E. coli; Fecal microbiota; Piglet; Resistance.

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