2. Academic Validation
  3. Polyanionic carbosilane dendrimers prevent hepatitis C virus infection in cell culture

Polyanionic carbosilane dendrimers prevent hepatitis C virus infection in cell culture

  • Nanomedicine. 2017 Jan;13(1):49-58. doi: 10.1016/j.nano.2016.08.018.
Daniel Sepúlveda-Crespo 1,  José Luis Jiménez 2,  Rafael Gómez 3,  Francisco Javier De La Mata 3,  Pedro L Majano 4,  Ma Ángeles Muñoz-Fernández 5,  Pablo Gastaminza 6
Affiliations

Affiliations

  • 1 Laboratorio InmunoBiología Molecular, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM), Spanish HIV-HGM BioBank, Madrid, Spain; Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain; Plataforma de Laboratorio, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
  • 2 Plataforma de Laboratorio, Hospital General Universitario Gregorio Marañón, Madrid, Spain; CIBER-BBN, Instituto de Salud Carlos III, Madrid, Spain.
  • 3 Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain; Departamento de Química Orgánica y Química Inorgánica, Universidad de Alcalá, Campus Universitario, Alcalá de Henares, Madrid, Spain; CIBER-BBN, Instituto de Salud Carlos III, Madrid, Spain.
  • 4 Molecular Biology Unit, Hospital Universitario de la Princesa, Instituto de Investigación Sanitaria Princesa (IIS-IP), Madrid, Spain; CIBERehd, Instituto de Salud Carlos III, Madrid, Spain.
  • 5 Laboratorio InmunoBiología Molecular, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM), Spanish HIV-HGM BioBank, Madrid, Spain; Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain; Plataforma de Laboratorio, Hospital General Universitario Gregorio Marañón, Madrid, Spain; CIBER-BBN, Instituto de Salud Carlos III, Madrid, Spain.
  • 6 Centro Nacional De Biotecnología-Consejo Superior de Investigaciones Científicas (CNB-CSIC), Campus Cantoblanco, Madrid, Spain. Electronic address: [email protected].
PMID: 27562210 DOI: 10.1016/j.nano.2016.08.018
Abstract

Hepatitis C virus (HCV) Infection is a major biomedical problem worldwide. Although new direct Antiviral agents (DAAs) have been developed for the treatment of chronic HCV Infection, the potential emergence of resistant virus variants and the difficulties to implement their administration worldwide make the development of novel Antiviral agents an urgent need. Moreover, no effective vaccine is available against HCV and transmission of the virus still occurs particularly when prophylactic measures are not taken. We used a cell-based system to screen a battery of polyanionic carbosilane dendrimers (PCDs) to identify compounds with Antiviral activity against HCV and show that they inhibit effective virus adsorption of major HCV genotypes. Interestingly, one of the PCDs irreversibly destabilized infectious virions. This compound displays additive effect in combination with a clinically relevant DAA, sofosbuvir. Our results support further characterization of these molecules as nanotools for the control of hepatitis C virus spread.

Keywords

Antiviral; Carbosilane dendrimer; Hepatitis C virus; Nanotechnology; Virucidal.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-15005
    99.97%, HCV Inhibitor
    HCV