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Sofosbuvir

(Synonyms: GS-7977; PSI-7977)

118 Cited Publications

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Based on 118 publication(s) in Google Scholar

Sofosbuvir (GS-7977) is an HCV RNA replication inhibitor with an EC₅₀ of 92 nM.

For research use only. We do not sell to patients.

Purity: 99.97%
CAS No.: 1190307-88-0
Formula: C₂₂H₂₉FN₃O₉P
Molecular Weight:529.45

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Sofosbuvir Chemical Structure

CAS No.: 1190307-88-0

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- Clinical Trial
Chemical Information
Publications (118)
Solvent & Solubility
- In Vitro
- In Vivo
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Bioz

Biological Activity

Description

Sofosbuvir (GS-7977) is an HCV RNA replication inhibitor with an EC₅₀ of 92 nM^[1].

IC₅₀ & Target

EC50: 92±5 nM (HCV)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
Calu-3	CC₅₀	512 μM Compound: Sofosbuvir	Cytotoxicity against human Calu-3 cells assessed as cell growth inhibition incubated for 4 hrs by XTT assay Cytotoxicity against human Calu-3 cells assessed as cell growth inhibition incubated for 4 hrs by XTT assay	[PMID: 36965227]
Cardiac muscle cell	CC₅₀	>300 μM Compound: SOF	Cardiotoxicity against human embryonic stem cell-induced ventricular cardiomyocytes administered on day 7 and measured on day 10 after 30 mins incubation by CellTiter Glo assay Cardiotoxicity against human embryonic stem cell-induced ventricular cardiomyocytes administered on day 7 and measured on day 10 after 30 mins incubation by CellTiter Glo assay	[PMID: 30653317]
CCRF-CEM	CC₅₀	>100 μM Compound: 1; SOF	Cytotoxicity against human CEM cells assessed as inhibition of cell proliferation after 4 days by MTT assay Cytotoxicity against human CEM cells assessed as inhibition of cell proliferation after 4 days by MTT assay	[PMID: 28595015]
CCRF-CEM	CC₅₀	>100 μM Compound: Sofosbuvir	Cytotoxicity against human CEM cells assessed as reduction in cell viability after 6 days by trypan blue exclusion assay Cytotoxicity against human CEM cells assessed as reduction in cell viability after 6 days by trypan blue exclusion assay	[PMID: 29066308]
CCRF-CEM	CC₅₀	>100 μM Compound: SOF	Cytotoxicity against human CCRF-CEM assessed reduction in cell viability after 4 days by MTT assay Cytotoxicity against human CCRF-CEM assessed reduction in cell viability after 4 days by MTT assay	[PMID: 30653317]
CCRF-CEM	CC₅₀	>100 μM Compound: SOF; 1	Cytotoxicity against human CEM cells after 6 days by trypan blue exclusion method Cytotoxicity against human CEM cells after 6 days by trypan blue exclusion method	[PMID: 30655167]
HepaRG	CC₅₀	44 μM Compound: SOF	Cytotoxicity in human HepaRG cells assessed as reduction in cell viability incubated for 14 days by CellTiter-Glo assay Cytotoxicity in human HepaRG cells assessed as reduction in cell viability incubated for 14 days by CellTiter-Glo assay	[PMID: 30653317]
Hepatocyte	CC₅₀	>300 μM Compound: SOF	Cytotoxicity against human primary hepatocytes assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human primary hepatocytes assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 30653317]
HepG2	IC₅₀	>50 μM Compound: 1; SOF	Cytotoxicity against human HepG2 cells assessed as inhibition of nuclear DNA levels measured on day 14 by RT-PCR method Cytotoxicity against human HepG2 cells assessed as inhibition of nuclear DNA levels measured on day 14 by RT-PCR method	[PMID: 28595015]
HepG2	IC₅₀	>50 μM Compound: 1; SOF	Mitochondrial toxicity in human HepG2 cells assessed as inhibition of cytochrome c oxidase subunit 2 DNA levels measured on day 14 by RT-PCR method Mitochondrial toxicity in human HepG2 cells assessed as inhibition of cytochrome c oxidase subunit 2 DNA levels measured on day 14 by RT-PCR method	[PMID: 28595015]
HepG2	CC₅₀	>50 μM Compound: Sofosbuvir	Cytotoxicity against human HepG2 cells after 96 hrs by CCK-8 assay Cytotoxicity against human HepG2 cells after 96 hrs by CCK-8 assay	[PMID: 29801997]
HepG2	IC₅₀	>50 μM Compound: SOF	Mitochondrial toxicity in human HepG2 cells assessed as inhibition of cytochrome c oxidase subunit 2 DNA level followed by medium plus drugs replenishment every 3 to 4 days for 14 days relative to control Mitochondrial toxicity in human HepG2 cells assessed as inhibition of cytochrome c oxidase subunit 2 DNA level followed by medium plus drugs replenishment every 3 to 4 days for 14 days relative to control	[PMID: 30653317]
HepG2	IC₅₀	45 μM Compound: SOF	Mitochondrial toxicity in human HepG2 cells assessed as inhibition of nuclear ribosomal-DNA level followed by medium plus drugs replenishment every 3 to 4 days for 14 days relative to control Mitochondrial toxicity in human HepG2 cells assessed as inhibition of nuclear ribosomal-DNA level followed by medium plus drugs replenishment every 3 to 4 days for 14 days relative to control	[PMID: 30653317]
HK-2	CC₅₀	>300 μM Compound: SOF	Nephrotoxicity in human HK2 cells after 72 hrs by Hoechst 33342/HCS live/dead green dye-based assay Nephrotoxicity in human HK2 cells after 72 hrs by Hoechst 33342/HCS live/dead green dye-based assay	[PMID: 30653317]
Huh-7	EC₅₀	0.254 μM Compound: 25	Antiviral activity against Hepatitis C virus genotype 1b in human Huh7-luc clone ET replicon cells assessed as inhibition of replicon replication after 3 days by luciferase assay Antiviral activity against Hepatitis C virus genotype 1b in human Huh7-luc clone ET replicon cells assessed as inhibition of replicon replication after 3 days by luciferase assay	[PMID: 24345201]
Huh-7	CC₅₀	>100 μM Compound: PSI-7977	Cytotoxicity against human Huh7.5.1 cells after 48 hrs by luciferase reporter gene assay Cytotoxicity against human Huh7.5.1 cells after 48 hrs by luciferase reporter gene assay	[PMID: 27994763]
Huh-7	EC₅₀	0.12 μM Compound: PSI-7977	Antiviral activity against HCV JFH-1 infected in human HuH7.5.1 cells after 48 hrs by luciferase reporter gene assay Antiviral activity against HCV JFH-1 infected in human HuH7.5.1 cells after 48 hrs by luciferase reporter gene assay	[PMID: 27994763]
Huh-7	CC₅₀	>10 μM Compound: 1; SOF	Cytotoxicity against human HuH7 cells assessed as inhibition of cell proliferation after 4 days by MTS assay Cytotoxicity against human HuH7 cells assessed as inhibition of cell proliferation after 4 days by MTS assay	[PMID: 28595015]
Huh-7	CC₅₀	>10 μM Compound: Sofosbuvir	Cytotoxicity against human HuH7 cells assessed as reduction in cell viability by MTT assay Cytotoxicity against human HuH7 cells assessed as reduction in cell viability by MTT assay	[PMID: 29066308]
Huh-7	CC₅₀	>1000 μM Compound: 17	Cytotoxicity against human HuH7 cells after 72 hrs by CellTiter 96 AQueous one solution cell proliferation assay Cytotoxicity against human HuH7 cells after 72 hrs by CellTiter 96 AQueous one solution cell proliferation assay	[PMID: 29172078]
Huh-7	CC₅₀	>100 μM Compound: SOF	Cytotoxicity against human HuH7 cells assessed reduction in cell viability after 3 to 5 days by MTT assay Cytotoxicity against human HuH7 cells assessed reduction in cell viability after 3 to 5 days by MTT assay	[PMID: 30653317]
Huh-7	CC₅₀	>100 μM Compound: SOF; 1	Cytotoxicity against human HuH7 cells assessed as reduction in rRNA levels after 5 days by RT-PCR analysis Cytotoxicity against human HuH7 cells assessed as reduction in rRNA levels after 5 days by RT-PCR analysis	[PMID: 30655167]
Huh-7	CC₅₀	>20 μM Compound: Sofosbuvir	Cytotoxicity against human HuH7 cells infected with HCV genotype 1b replicon after 72 hrs by CellTiter-Fluor reagent based fluorescence assay Cytotoxicity against human HuH7 cells infected with HCV genotype 1b replicon after 72 hrs by CellTiter-Fluor reagent based fluorescence assay	[PMID: 30683552]
Huh-7	EC₅₀	0.053 μM Compound: 1	Antiviral activity against HCV genotype 1b Con1 over expressing CES1 infected in human HuH7 replicon cells assessed as inhibition of viral replication after 3 days by luciferase reporter gene assay Antiviral activity against HCV genotype 1b Con1 over expressing CES1 infected in human HuH7 replicon cells assessed as inhibition of viral replication after 3 days by luciferase reporter gene assay	[PMID: 31740203]
Huh-7	EC₅₀	0.062 μM Compound: 1	Antiviral activity against HCV genotype 1b Con1 expressing wild type NS5B infected in human HuH7 replicon cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene based assay Antiviral activity against HCV genotype 1b Con1 expressing wild type NS5B infected in human HuH7 replicon cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene based assay	[PMID: 31740203]
Huh-7	EC₅₀	0.0751 μM Compound: 1	Antiviral activity against HCV genotype 3a expressing wild type NS5B infected in human HuH7 replicon cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene based assay Antiviral activity against HCV genotype 3a expressing wild type NS5B infected in human HuH7 replicon cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene based assay	[PMID: 31740203]
Huh-7	EC₅₀	0.0785 μM Compound: 1	Antiviral activity against HCV genotype 4a expressing wild type NS5B infected in human HuH7 replicon cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene based assay Antiviral activity against HCV genotype 4a expressing wild type NS5B infected in human HuH7 replicon cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene based assay	[PMID: 31740203]
Huh-7	EC₅₀	0.0867 μM Compound: 1	Antiviral activity against HCV genotype 6a expressing wild type NS5B infected in human HuH7 replicon cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene based assay Antiviral activity against HCV genotype 6a expressing wild type NS5B infected in human HuH7 replicon cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene based assay	[PMID: 31740203]
Huh-7	EC₅₀	0.155 μM Compound: 1	Antiviral activity against HCV genotype 1a H77 infected in human HuH7 replicon cells assessed as inhibition of viral replication after 3 days by luciferase reporter gene assay Antiviral activity against HCV genotype 1a H77 infected in human HuH7 replicon cells assessed as inhibition of viral replication after 3 days by luciferase reporter gene assay	[PMID: 31740203]
Huh-7	EC₅₀	0.188 μM Compound: 1	Antiviral activity against HCV genotype 1b Con1 infected in human HuH7 replicon cells assessed as inhibition of viral replication after 3 days by luciferase reporter gene assay Antiviral activity against HCV genotype 1b Con1 infected in human HuH7 replicon cells assessed as inhibition of viral replication after 3 days by luciferase reporter gene assay	[PMID: 31740203]
Huh-7	EC₅₀	0.3301 μM Compound: 1	Antiviral activity against HCV genotype 3a expressing NS5B S282T mutant infected in human HuH7 replicon cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene based assay Antiviral activity against HCV genotype 3a expressing NS5B S282T mutant infected in human HuH7 replicon cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene based assay	[PMID: 31740203]
Huh-7	EC₅₀	0.6376 μM Compound: 1	Antiviral activity against HCV genotype 1b Con1 expressing NS5B S282T mutant infected in human HuH7 replicon cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene based assay Antiviral activity against HCV genotype 1b Con1 expressing NS5B S282T mutant infected in human HuH7 replicon cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene based assay	[PMID: 31740203]
Huh-7	EC₅₀	0.6813 μM Compound: 1	Antiviral activity against HCV genotype 2a expressing wild type NS5B infected in human HuH7 replicon cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene based assay Antiviral activity against HCV genotype 2a expressing wild type NS5B infected in human HuH7 replicon cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene based assay	[PMID: 31740203]
Huh-7	EC₅₀	1.002 μM Compound: 1	Antiviral activity against HCV genotype 2a expressing NS5B S282T mutant infected in human HuH7 replicon cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene based assay Antiviral activity against HCV genotype 2a expressing NS5B S282T mutant infected in human HuH7 replicon cells assessed as inhibition of viral replication after 4 days by luciferase reporter gene based assay	[PMID: 31740203]
Huh-7	EC₅₀	0.155 μM Compound: Sofosbuvir	Inhibition of NS5B polymerase in HCV genotype 1a H77 infected in human HuH7 replicon cells assessed as inhibition of viral replication after 3 days by luciferase reporter gene assay Inhibition of NS5B polymerase in HCV genotype 1a H77 infected in human HuH7 replicon cells assessed as inhibition of viral replication after 3 days by luciferase reporter gene assay	[PMID: 32046903]
Huh-7	EC₅₀	0.23 μM Compound: Sofosbuvir	Inhibition of NS5B polymerase in HCV genotype 1b Con1 infected in human HuH7 replicon cells assessed as inhibition of viral replication after 3 days by luciferase reporter gene assay Inhibition of NS5B polymerase in HCV genotype 1b Con1 infected in human HuH7 replicon cells assessed as inhibition of viral replication after 3 days by luciferase reporter gene assay	[PMID: 32046903]
Huh-7	CC₅₀	200 μM Compound: Sofosbuvir	Cytotoxicity against human HuH7 cells assessed as reduction in cell viability by measuring ATP level measured after 72 hrs by CellTiter-Glo luminescent assay Cytotoxicity against human HuH7 cells assessed as reduction in cell viability by measuring ATP level measured after 72 hrs by CellTiter-Glo luminescent assay	[PMID: 32435411]
Huh-7	IC₅₀	3.8 μM Compound: Sofosbuvir	Antiviral activity against Dengue virus serotype 2 New Guinea C infected at 50 TCID50 in human HuH7 cells assessed as inhibition of viral replication and 72 hrs later re-infected pre-seeded HuH7 cells with viral supernatant collected from previous infecte Antiviral activity against Dengue virus serotype 2 New Guinea C infected at 50 TCID50 in human HuH7 cells assessed as inhibition of viral replication and 72 hrs later re-infected pre-seeded HuH7 cells with viral supernatant collected from previous infecte	[PMID: 32435411]
Huh-7	CC₅₀	200 μM Compound: Sofosbuvir	Cytotoxicity against human Huh-7 cells assessed as reduction in cell viability by Celltiter-Glo luminescent cell viability assay Cytotoxicity against human Huh-7 cells assessed as reduction in cell viability by Celltiter-Glo luminescent cell viability assay	[PMID: 34273661]
Huh-7	CC₅₀	>100 μM Compound: SOF	Cytotoxicity against human Huh-7 cells assessed as reduction in cell viability measured after 24 hrs by viral-Tox-glo assay Cytotoxicity against human Huh-7 cells assessed as reduction in cell viability measured after 24 hrs by viral-Tox-glo assay	[PMID: 37159959]
Huh-7.5	CC₅₀	>200 μM Compound: SOF	Cytotoxicity against human Huh7.5 cells assessed as reduction in cell viability incubated for 96 hrs by MTT assay Cytotoxicity against human Huh7.5 cells assessed as reduction in cell viability incubated for 96 hrs by MTT assay	[PMID: 35050619]
Huh-7.5	CC₅₀	121.47 μM Compound: SOF	Cytotoxicity against human Huh7.5 cells carrying HCV subgenomic replicons assessed as reduction in cell viability incubated for 96 hrs by MTT assay Cytotoxicity against human Huh7.5 cells carrying HCV subgenomic replicons assessed as reduction in cell viability incubated for 96 hrs by MTT assay	[PMID: 35050619]
Huh-7.5	CC₅₀	170.85 μM Compound: SOF	Cytotoxicity against human Huh7.5 cells infected with HCVcc assessed as reduction in cell viability incubated for 96 hrs by MTT assay Cytotoxicity against human Huh7.5 cells infected with HCVcc assessed as reduction in cell viability incubated for 96 hrs by MTT assay	[PMID: 35050619]
Huh-7.5	CC₅₀	>200 μM Compound: Sofosbuvir	Cytotoxicity against human Huh7.5 cells assessed as reduction in cell viability by CCK-8 assay Cytotoxicity against human Huh7.5 cells assessed as reduction in cell viability by CCK-8 assay	[PMID: 38561238]
PBMC	CC₅₀	>100 μM Compound: 1; SOF	Cytotoxicity against human PBMC assessed as inhibition of cell proliferation after 5 days by MTT assay Cytotoxicity against human PBMC assessed as inhibition of cell proliferation after 5 days by MTT assay	[PMID: 28595015]
PBMC	CC₅₀	>100 μM Compound: Sofosbuvir	Cytotoxicity against human PBMC assessed as reduction in cell viability after 6 days by trypan blue exclusion assay Cytotoxicity against human PBMC assessed as reduction in cell viability after 6 days by trypan blue exclusion assay	[PMID: 29066308]
PBMC	CC₅₀	>100 μM Compound: SOF	Cytotoxicity against human PBMC assessed reduction in cell viability after 5 days by MTT assay Cytotoxicity against human PBMC assessed reduction in cell viability after 5 days by MTT assay	[PMID: 30653317]
PBMC	CC₅₀	>100 μM Compound: SOF; 1	Cytotoxicity against human PBMC after 6 days by trypan blue exclusion method Cytotoxicity against human PBMC after 6 days by trypan blue exclusion method	[PMID: 30655167]
Vero	CC₅₀	>100 μM Compound: 1; SOF	Cytotoxicity against African green monkey Vero cells assessed as inhibition of cell proliferation after 3 days by MTT assay Cytotoxicity against African green monkey Vero cells assessed as inhibition of cell proliferation after 3 days by MTT assay	[PMID: 28595015]
Vero	CC₅₀	>100 μM Compound: Sofosbuvir	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 6 days by trypan blue exclusion assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 6 days by trypan blue exclusion assay	[PMID: 29066308]
Vero	CC₅₀	>100 μM Compound: SOF	Cytotoxicity against African green monkey Vero cells assessed reduction in cell viability after 3 days by MTT assay Cytotoxicity against African green monkey Vero cells assessed reduction in cell viability after 3 days by MTT assay	[PMID: 30653317]
Vero	CC₅₀	>100 μM Compound: SOF; 1	Cytotoxicity against African green monkey Vero cells after 3 days by hemocytometric method Cytotoxicity against African green monkey Vero cells after 3 days by hemocytometric method	[PMID: 30655167]
Vero	CC₅₀	360 μM Compound: 5	Cytotoxicity against African green monkey Vero cells Cytotoxicity against African green monkey Vero cells	[PMID: 31549836]
Vero	EC₅₀	30.9 μM Compound: 5	Inhibition of RNA-dependent RNA polymerase in Zika virus infected in African green monkey Vero cells assessed as antiviral activity by DAPI-staining based assay Inhibition of RNA-dependent RNA polymerase in Zika virus infected in African green monkey Vero cells assessed as antiviral activity by DAPI-staining based assay	[PMID: 31549836]

In Vitro

When cathepsin A (CatA) is incubated with PSI-7977 or Sofosbuvir (PSI-7977) for 150 min, ~18-fold more PSI-352707 is formed when Sofosbuvir (PSI-7977) is the substrate compared with PSI-7976. Moreover, the catalytic efficiency for Sofosbuvir (PSI-7977) with CatA is ~30-fold higher than that for PSI-7976^[1]. The genotype coverage of Sofosbuvir (PSI-7977) by using GT 1b (Con1)-, 1a (H77)-, and 2a (JFH-1)-derived replicons and GT 1b chimeric replicons containing the NS5B region from the J6 GT 2a isolate and from GT 2b and GT 3a patient isolates is evaluated, Sofosbuvir (PSI-7977) inhibits the replication of these replicons with similar EC₅₀s (between 16 and 48 nM), and is especially active against the chimeric replicon containing the J6 NS5B (EC₅₀=4.7 nM). Sofosbuvir (PSI-7977) inhibits clone A (GT 1b) wild-type and S282T replicons with EC₉₀ values of 0.42 and 7.8 μM, respectively^[2]. In the clone A replicon assay, Sofosbuvir (PSI-7977) produces anti-HCV activity with EC₉₀ values 0.42 μM^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 1190307-88-0
Appearance Solid
Molecular Weight 529.45
Formula C₂₂H₂₉FN₃O₉P
Color White to off-white
SMILES

C[C@@]1(F)[C@H](O)[C@@H](CO[P@](OC2=CC=CC=C2)(N[C@@H](C)C(OC(C)C)=O)=O)O[C@H]1N(C=CC(N3)=O)C3=O
Synonyms

GS-7977; PSI-7977
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (118)

All Microbiological AssayBio/Physico-chemical AssayRT-PCRWB

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Journal Impact Factor

Nat Med

Regulation of the hepatitis C virus RNA replicase by endogenous lipid peroxidation. [Abstract]

2014 Aug;20(8):927-35. PMID: 25064127
Cell

Structural basis for human Cav1.2 inhibition by multiple drugs and the neurotoxin calciseptine. [Abstract]

2023 Nov 22;186(24):5363-5374.e16. PMID: 37972591
Cell

Structural basis for the severe adverse interaction of sofosbuvir and amiodarone on L-type Cav channels. [Abstract]

2022 Dec 8;185(25):4801-4810.e13. PMID: 36417914
Nat Immunol

NLRX1 promotes immediate IRF1-directed antiviral responses by limiting dsRNA-activated translational inhibition mediated by PKR. [Abstract]

2017 Dec;18(12):1299-1309. PMID: 28967880
Gastroenterology

Hepatitis C virus infection induces autocrine interferon signaling by human liver endothelial cells and release of exosomes, which inhibits viral replication. [Abstract]

2015 Feb;148(2):392-402.e13. PMID: 25447848
Nat Microbiol

Basal expression of interferon regulatory factor 1 drives intrinsic hepatocyte resistance to multiple RNA viruses. [Abstract]

2019 Jul;4(7):1096-1104. PMID: 30988429
Nucleic Acids Res

CSNK2B modulates IRF1 binding to functional DNA elements and promotes basal and agonist-induced antiviral signaling. [Abstract]

2023 May 22;51(9):4451-4466. PMID: 37094077
Nucleic Acids Res

TNRC6 proteins modulate hepatitis C virus replication by spatially regulating the binding of miR-122/Ago2 complexes to viral RNA. [Abstract]

2019 Jul 9;47(12):6411-6424. PMID: 30997501

Sofosbuvir purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2019 Jul 9;47(12):6411-6424. [Abstract]

Positive-strand HCV RNA stability in cells following Sofosbuvir (10 μM) arrest of viral RNA synthesis. HCV RNA level at 0 h was arbitrarily set to 100%.
Nucleic Acids Res

Differential hepatitis C virus RNA target site selection and host factor activities of naturally occurring miR-122 3΄ variants. [Abstract]

2017 May 5;45(8):4743-4755. PMID: 28082397

Sofosbuvir purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2017 May 5;45(8):4743-4755. [Abstract]

PH5CH8 cells were co-electroporated with HCV JFH1/GLuc RNA and the (left) 23-3΄U variant or (right) 23-3΄Up6 mutant, then cultured in the presence or absence of the NS5B inhibitor sofosbuvir (SOF). As negative controls for viral RNA amplification, parallel cultures were co-electroporated with miR-124 or a non-replicating HCV RNA containing a lethal GND mutation in the NS5B polymerase. Secreted GLuc activity was followed at intervals.
Cell Rep Med

Human liver-derived organoids recapitulate Oropouche virus infection and manifestation, enabling antiviral drug discovery. [Abstract]

2026 Mar 9:102646. PMID: 41806841
Genomics Proteomics Bioinformatics

NSUN2-mediated HCV RNA m5C Methylation Facilitates Viral RNA Stability and Replication. [Abstract]

2025 May 10;23(1):qzaf008. PMID: 39957240

Sofosbuvir purchased from MedChemExpress. Usage Cited in: Genomics Proteomics Bioinformatics. 2025 May 10;23(1):qzaf008. [Abstract]

WB assays of the expression of NSUN2 as well as HCV NS3 and Core proteins in Huh7.5.1 cells treated with Sofosbuvir (0-10 μM) or in combination with SAH at the indicated concentrations at 6 h post infection, and then harvested at 72 h post infection.

Sofosbuvir purchased from MedChemExpress. Usage Cited in: Genomics Proteomics Bioinformatics. 2025 May 10;23(1):qzaf008. [Abstract]

WB assays of the expression of NSUN2 as well as HCV NS3 and Core proteins in Huh7.5.1 cells treated with Sofosbuvir (0-10 μM) or in combination with sinefungin at the indicated concentrations at 6 h post infection, and then harvested at 72 h post infection.
Br J Pharmacol

Betulinic acid exerts anti-hepatitis C virus activity via the suppression of NF-κB- and MAPK-ERK1/2-mediated COX-2 expression. [Abstract]

2015 Sep;172(18):4481-4492. PMID: 26102077

Sofosbuvir purchased from MedChemExpress. Usage Cited in: Br J Pharmacol. 2015 Sep;172(18):4481-4492. [Abstract]

Ava5 cells were treated with a combination of BA and IFN-α, telaprevir or sofosbuvir at various concentrations for 3 days. Total RNA was extracted and the levels of HCV RNA were quantified by qRT-PCR analysis.
Br J Pharmacol

Andrographolide exerts anti-hepatitis C virus activity by up-regulating haeme oxygenase-1 via the p38 MAPK/Nrf2 pathway in human hepatoma cells. [Abstract]

2014 Jan;171(1):237-52. PMID: 24117426
JHEP Rep

Dynamic evolution of the sofosbuvir-associated variant A1343V in HEV-infected patients under concomitant sofosbuvir-ribavirin treatment. [Abstract]

2024 Jan 3;6(3):100989. PMID: 38434938
Biomed Pharmacother

A proof-of-concept study in HCV-infected Huh7.5 cells for shortening the duration of DAA-based triple treatment regimens. [Abstract]

2019 Aug:116:108976. PMID: 31103827
J Med Chem

2-((4-Arylpiperazin-1-yl)methyl)benzonitrile Derivatives as Orally Available Inhibitors of Hepatitis C Virus with a Novel Mechanism of Action. [Abstract]

2020 Jun 11;63(11):5972-5989. PMID: 32378892
J Med Chem

Design and Synthesis of Cajanine Analogues against Hepatitis C Virus through Down-Regulating Host Chondroitin Sulfate N-Acetylgalactosaminyltransferase 1. [Abstract]

2016 Nov 23;59(22):10268-10284. PMID: 27783522

Sofosbuvir purchased from MedChemExpress. Usage Cited in: J Med Chem. 2016 Nov 23;59(22):10268-10284. [Abstract]

Huh7.5 cells are infected with HCV (45 IU/cell) and simultaneously treated with Simeprevir (A, 0.025 μM), Sofosbuvir (B, 0.1 μM), or Daclatasvir (C, 16 pM) alone or with 1 (6.25 μM).
Int J Radiat Oncol Biol Phys

Targeting Phosphatidylinositol 4-Kinase IIIα for Radiosensitization: A Potential Model of Drug Repositioning Using an Anti-Hepatitis C Viral Agent. [Abstract]

2016 Nov 15;96(4):867-876. PMID: 27788957
EMBO Rep

Long noncoding RNA EGOT negatively affects the antiviral response and favors HCV replication. [Abstract]

2016 Jul;17(7):1013-28. PMID: 27283940
Eur J Med Chem

System-oriented optimization of multi-target 2,6-diaminopurine derivatives: Easily accessible broad-spectrum antivirals active against flaviviruses, influenza virus and SARS-CoV-2. [Abstract]

2021 Nov 15:224:113683. PMID: 34273661
Eur J Med Chem

Discovery and evolution of aloperine derivatives as a new family of HCV inhibitors with novel mechanism. [Abstract]

2018 Jan 1:143:1053-1065. PMID: 29232582

Sofosbuvir purchased from MedChemExpress. Usage Cited in: Eur J Med Chem. 2018 Jan 1:143:1053-1065. [Abstract]

GS4.3 cells are treated with 7f (20 μM), or Telaprevir (0.5μM), Sofosbuvir (0.8 μM), Daclatasvir (0.15 μM) or solvent control for 6 days.

Sofosbuvir purchased from MedChemExpress. Usage Cited in: Eur J Med Chem. 2018 Jan 1:143:1053-1065. [Abstract]

Huh7.5 cells are infected with HCV and simultaneously treated with 7f (10 μM) or DAA (0.04 μM Simeprevir, 0.08 μM Sofosbuvir, or 16 pM Daclatasvir) or 7f plus DAA.
Pharmaceutics

Activation of Tenofovir Alafenamide and Sofosbuvir in the Human Lung and Its Implications in the Development of Nucleoside/Nucleotide Prodrugs for Treating SARS-CoV-2 Pulmonary Infection. [Abstract]

2021 Oct 11;13(10):1656. PMID: 34683949
J Gastroenterol

Combinations of two drugs among NS3/4A inhibitors, NS5B inhibitors and non-selective antiviral agents are effective for hepatitis C virus with NS5A-P32 deletion in humanized-liver mice. [Abstract]

2019 May;54(5):449-458. PMID: 30684016
Cells

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2023 Dec;95(12):e29290. PMID: 38102947
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2018 Nov 30;3(1):160-172. PMID: 30620002
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2017 Jul 13;1(6):550-563. PMID: 29404478
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2017 Jan;13(1):49-58. PMID: 27562210
Int J Antimicrob Agents

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2020 Dec 16;65(1):e01508-20. PMID: 33122171
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2017 Jun 19:8:1129. PMID: 28674529
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2014 Aug;58(8):4555-64. PMID: 24867984
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2014 Jun;58(6):3327-34. PMID: 24687498
Antimicrob Agents Chemother

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2013 Mar;57(3):1180-91. PMID: 23254429
Ann Hepatol

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Nov-Dec 2019;18(6):816-824. PMID: 31594756
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2021 Dec;102(12). PMID: 34949310
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TGF-β isoforms inhibit hepatitis C virus propagation in transforming growth factor beta/SMAD protein signalling pathway dependent and independent manners. [Abstract]

2021 Apr;25(7):3498-3510. PMID: 33682288
Antiviral Res

Substitutions M478K and A482G in the polymerase of yellow fever virus confer resistance to sofosbuvir and uprifosbuvir in vitro. [Abstract]

2026 Feb 27:106381. PMID: 41765050
Antiviral Res

Effectiveness of nucleoside analogs against Wetland virus infection. [Abstract]

2025 Apr:236:106114. PMID: 39954869
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Development of a reporter HBoV1 strain for antiviral drug screening and life cycle studies. [Abstract]

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Identification of a novel replication-competent hepatitis C virus variant that confers the sofosbuvir resistance. [Abstract]

2022 Jan:197:105224. PMID: 34864126
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2020 May;177:104734. PMID: 32057770
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2020 Mar;175:104708. PMID: 31931104
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2019 Oct;170:104570. PMID: 31362004
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2017 Dec:148:5-14. PMID: 29074218
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2017 Oct:146:191-200. PMID: 28935193
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2017 Mar;139:18-24. PMID: 28025084
Antiviral Res

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2016 Aug:132:287-95. PMID: 27387825
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2013 Jul;99(1):6-11. PMID: 23660623
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2025 Aug 2;15(1):28197. PMID: 40750837
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5-Oxo-1-[(2,3,6,7-tetramethoxy-9-phenanthrenyl)methyl]-L-proline Inhibits Hepatitis C Virus Entry. [Abstract]

2019 May 13;9(1):7288. PMID: 31086268
Sci Rep

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2019 Apr 5;9(1):5722. PMID: 30952914
Sci Rep

Lobohedleolide suppresses hepatitis C virus replication via JNK/c-Jun-C/EBP-mediated down-regulation of cyclooxygenase-2 expression. [Abstract]

2018 Jun 6;8(1):8676. PMID: 29875371
Sci Rep

Effects of Resistance-Associated NS5A Mutations in Hepatitis C Virus on Viral Production and Susceptibility to Antiviral Reagents. [Abstract]

2016 Oct 5;6:34652. PMID: 27703205
Mol Divers

Repurposed drugs as PCSK9-LDLR disruptors for lipid lowering and cardiovascular disease therapeutics. [Abstract]

2024 Dec 8. PMID: 39645639
J Virol

Elevation of Plasminogen Activator Inhibitor-1 promotes differentiation of Cancer Stem-like Cell state by Hepatitis C Virus infection. [Abstract]

2021 Apr 26;95(10):e02057-20. PMID: 33627392
J Virol

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2014 May;88(10):5578-94. PMID: 24599999
Viruses

Identification and Tracking of Antiviral Drug Combinations. [Abstract]

2020 Oct 18;12(10):1178. PMID: 33080984
Viruses

Generation of Combinatorial Lentiviral Vectors Expressing Multiple Anti-Hepatitis C Virus shRNAs and Their Validation on a Novel HCV Replicon Double Reporter Cell Line. [Abstract]

2020 Sep 18;12(9):1044 PMID: 32962117
Viruses

Potential Therapeutic Agents for Feline Calicivirus Infection. [Abstract]

2018 Aug 16;10(8). pii: E433. PMID: 30115859
Hepatol Res

Elevated serum uric acid level was a notable adverse event during combination therapy with sofosbuvir and ribavirin. [Abstract]

2018 Feb;48(3):E347-E353. PMID: 28834004
Chem Biol Drug Des

Sofosbuvir as treatment against dengue?. [Abstract]

2018 Feb;91(2):448-455. PMID: 28834304
Bioorg Med Chem

Harzianoic acids A and B, new natural scaffolds with inhibitory effects against hepatitis C virus. [Abstract]

2019 Feb 1;27(3):560-567. PMID: 30606673
J Chromatogr B Analyt Technol Biomed Life Sci

Quantification of second generation direct-acting antivirals daclatasvir, elbasvir, grazoprevir, ledipasvir, simeprevir, sofosbuvir and velpatasvir in human plasma by UPLC-MS/MS. [Abstract]

2019 Mar 15:1110-1111:15-24. PMID: 30776611
SLAS Discov

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Comparative analysis of different cell systems for Zika virus (ZIKV) propagation and evaluation of anti-ZIKV compounds in vitro. [Abstract]

2018 Jan 15:244:64-70. PMID: 29113824
Virus Res

Evaluation of preclinical antimalarial drugs, which can overcome direct-acting antivirals-resistant hepatitis C viruses, using the viral reporter assay systems. [Abstract]

2017 May 2:235:37-48. PMID: 28322919
PLoS One

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PLoS One

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Transpl Infect Dis

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PLoS One

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2016 Jul 21;11(7):e0159511. PMID: 27442520
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2016 Mar 29;11(3):e0152236. PMID: 27023634
Biomed Res Int

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Sofosbuvir purchased from MedChemExpress. Usage Cited in: Biomed Res Int. 2017:2017:1236801. [Abstract]

Huh7.5 (HCV+) cells are treated with 1 μM of Sofosbuvir or solvent control. At 24 hours, the cells are washed and continuously incubated with fresh culture media containing drugs again for 48 hours. The cultural supernatants are then harvested and directly incubated to naïve Huh7.5 cells. After been passaged 1~3 times, the newly infected cells are treated with 1 μM of Sofosbuvir for 72 hours. Intracellular proteins are extracted and detected with WB.
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2017 Feb;31(3):341-346. PMID: 27825248
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bioRxiv

2026 Feb 4.
bioRxiv

Drug-induced phospholipidosis as an artifact in antiviral drug repurposing. [Abstract]

2025 Nov 21:2025.11.20.689520. PMID: 41332603
bioRxiv

2025 August 15.
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2025.
bioRxiv

Combinations of approved oral nucleoside analogues confer potent suppression of alphaviruses in vitro and in vivo. [Abstract]

2025 Jan 25:2025.01.24.633564. PMID: 39896535
University of Colorado Denver

2024.
Preprints

2024 Feb 19.
SSRN

2024 Jan 23.
bioRxiv

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2023 Aug 20:2023.08.19.553982. PMID: 37645728
SSRN

2023 Mar 30.
Norwegian University of Science and Technology

2021 Oct.
bioRxiv

2020 May.
bioRxiv

2020 Apr.
University Estadual De Campinas Institudo De Biologia

2019 Sep.
bioRxiv

2019 Aug.
Charles University

2019 Jun.
Charles University

2019 Jun.
Seoul National University

2016 Aug.
Universidad Autónoma de Madrid

2016 Jan 12.
Open Virol J

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2014 Mar 7;8:1-8. PMID: 24799968

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (94.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 25 mg/mL (47.22 mM; ultrasonic and warming and heat to 50°C)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.8888 mL

9.4438 mL

18.8875 mL

5 mM

0.3778 mL

1.8888 mL

3.7775 mL

10 mM

0.1889 mL

0.9444 mL

1.8888 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 1.67 mg/mL (3.15 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 1.67 mg/mL (3.15 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 1.67 mg/mL (3.15 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 4.55 mg/mL (8.59 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Protocol

Cell Assay^[1]

Clone A cells are seeded into T75 flasks at about 5×10⁶ cells/flask in Dulbecco's modified Eagle's medium (DMEM) containing 100 IU/mL Penicillin/100 μg/mL streptomycin and 10% fetal bovine serum. Similarly, human primary hepatocytes are seeded in cell plating medium into T75 flasks at about 5×10⁶ cells/flask. After overnight incubation to allow the cells to attach, cells are incubated with 50 μM PSI-7851, PSI-7976, or Sofosbuvir (PSI-7977) in fresh medium for clone A cells or in cell maintenance medium for primary hepatocytes for up to 24 h at 37°C in a 5% CO₂ atmosphere. The same procedures are applied when radiolabeled PSI-7851 is used in the study except that 1×10⁶ cells per well are seeded into a 6-well plate, and the cells are incubated with 5 μM [³H]PSI-7851. At selected times, the medium is removed, and the cell layer is washed with cold phosphate-buffered saline (PBS). After trypsinization, cells are counted and centrifuged at 1,200 rpm for 5 min. The cell pellets are suspended in 1 mL of cold 60% methanol and incubated overnight at −20°C. The samples are centrifuged at 14,000 rpm for 5 min, and the supernatants are collected and dried using a SpeedVac concentrator and stored at −20°C until they are analyzed by high performance liquid chromatography (HPLC). Residues are suspended in 100 μL of water, and 50-μL aliquots are injected into HPLC^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (285 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Murakami E, et al. Mechanism of activation of PSI-7851 and its diastereoisomer PSI-7977.J Biol Chem. 2010 Nov 5;285(45):34337-47. [Content Brief]

[2]. Lam AM, et al. Genotype and subtype profiling of PSI-7977 as a nucleotide inhibitor of hepatitis C virus. Antimicrob Agents Chemother. 2012 Jun;56(6):3359-68. [Content Brief]

[3]. Sofia MJ, et al. Discovery of a β-d-2'-deoxy-2'-α-fluoro-2'-β-C-methyluridine nucleotide prodrug (PSI-7977) for the treatment of hepatitis C virus. J Med Chem. 2010 Oct 14;53(19):7202-18. [Content Brief]

[4]. Zhang X, et al. Discovery and evolution of aloperine derivatives as a new family of HCV inhibitors with novel mechanism. Eur J Med Chem. 2018 Jan 1;143:1053-1065. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	1.8888 mL	9.4438 mL	18.8875 mL	47.2188 mL
	5 mM	0.3778 mL	1.8888 mL	3.7775 mL	9.4438 mL
	10 mM	0.1889 mL	0.9444 mL	1.8888 mL	4.7219 mL
	15 mM	0.1259 mL	0.6296 mL	1.2592 mL	3.1479 mL
	20 mM	0.0944 mL	0.4722 mL	0.9444 mL	2.3609 mL
	25 mM	0.0756 mL	0.3778 mL	0.7555 mL	1.8888 mL
	30 mM	0.0630 mL	0.3148 mL	0.6296 mL	1.5740 mL
	40 mM	0.0472 mL	0.2361 mL	0.4722 mL	1.1805 mL
DMSO	50 mM	0.0378 mL	0.1889 mL	0.3778 mL	0.9444 mL
	60 mM	0.0315 mL	0.1574 mL	0.3148 mL	0.7870 mL
	80 mM	0.0236 mL	0.1180 mL	0.2361 mL	0.5902 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

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MCE Japan Authorized Agent:

Product Name:
CAS No.:
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Purity:
Quantity:

Sofosbuvir

Anti-Infection Compound Library

FDA-Approved Drug Library

Anti-Cancer Compound Library

Antiviral Compound Library

Drug Repurposing Compound Library

Covalent Screening Library

Nucleotide Compound Library

Anti-COVID-19 Compound Library

NMPA-Approved Drug Library

Orally Active Compound Library

FDA Approved & Pharmacopeial Drug Library

Anti-Hepatitis C Virus Compound Library

Rare Diseases Drug Library

Pediatric Drug Library

EMA-Approved Drug Library

FDA-Approved Anticancer Drug Library

Non-steroidal Anti-Inflammatory Compound Library

Heterocyclic Compound Library

Off-patent Drug Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Covalent Screening Library Plus

Drug Repurposing Compound Library Plus

FDA-Approved Drug Library Plus

FDA-Approved Drug Library Mini

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Classic FDA-Approved Drug Library

Publications Citing Use of MedChemExpress (MCE) Sofosbuvir

Biological Activity

Chemical Information

Publications (118)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Sofosbuvir

Anti-Infection Compound Library

FDA-Approved Drug Library

Anti-Cancer Compound Library

Antiviral Compound Library

Drug Repurposing Compound Library

Covalent Screening Library

Nucleotide Compound Library

Anti-COVID-19 Compound Library

NMPA-Approved Drug Library

Orally Active Compound Library

FDA Approved & Pharmacopeial Drug Library

Anti-Hepatitis C Virus Compound Library

Rare Diseases Drug Library

Pediatric Drug Library

EMA-Approved Drug Library

FDA-Approved Anticancer Drug Library

Non-steroidal Anti-Inflammatory Compound Library

Heterocyclic Compound Library

Off-patent Drug Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Covalent Screening Library Plus

Drug Repurposing Compound Library Plus

FDA-Approved Drug Library Plus

FDA-Approved Drug Library Mini

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Classic FDA-Approved Drug Library

Publications Citing Use of MedChemExpress (MCE) Sofosbuvir

Biological Activity

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Cat. No.: HY-15005