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  3. A Fluorescent Probe for Neural Stem/Progenitor Cells with High Differentiation Capability into Neurons

A Fluorescent Probe for Neural Stem/Progenitor Cells with High Differentiation Capability into Neurons

  • Chembiochem. 2016 Nov 17;17(22):2118-2122. doi: 10.1002/cbic.201600490.
Beomsue Kim 1,  Suihan Feng 2,  Seong-Wook Yun 1,  Cheryl Leong 1,  Rudrakanta Satapathy 2,  Si Yan Diana Wan 1,  Young-Tae Chang 2 1
Affiliations

Affiliations

  • 1 Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Singapore, 138667, Singapore.
  • 2 Department of Chemistry and Medicinal Chemistry Programme, National University of Singapore, Singapore, 117543, Singapore.
PMID: 27782351 DOI: 10.1002/cbic.201600490
Abstract

Selection of a specific neural stem/progenitor cells (NSPCs) has attracted broad attention in regenerative medicine for neurological disorders. Here, we report a fluorescent probe, CDg13, and its application for isolating strong neurogenic NSPCs. In comparison to the NSPCs isolated by Other biomarkers, CDg13-stained NSPCs showed higher capability to differentiate into neurons. Target identification revealed that the fluorescence intensity of the probe within cells is inversely proportional to the expression levels of mouse and human Abcg2 transporters. These findings suggest that low Abcg2 expression is a biomarker for neurogenic NSPCs in mouse brain. Furthermore, CDg13 can be used to isolate Abcg2low cells from heterogeneous cell populations.

Keywords

ABC transporters; Abcg2; CDg13; cell recognition; fluorescent probes; neural stem/progenitor cells.

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