2. Others Membrane Transporter/Ion Channel
  3. Fluorescent Dye BCRP
  4. CDg13

CDg13 is a fluorescent probe that specifically detects living neural stem/progenitor cells. CDg13 localizes to the endoplasmic reticulum via its dihexyl moiety, with no interaction with endoplasmic reticulum biomolecules. CDg13 undergoes selective efflux by active ABCG2 transporters, with increased intracellular accumulation following ABCG2 inhibition or knockdown. CDg13 isolates and enriches self-renewable neural stem/progenitor cells from embryonic mouse brain tissue, stains neural stem/progenitor cells brightly, and sorts ABCG2low cell populations from heterogeneous populations. (Ex/Em = 520/553 nm)

For research use only. We do not sell to patients.

CDg13

CDg13 Chemical Structure

CAS No. : 1451048-96-6

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

CDg13 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

CDg13 is a fluorescent probe that specifically detects living neural stem/progenitor cells. CDg13 localizes to the endoplasmic reticulum via its dihexyl moiety, with no interaction with endoplasmic reticulum biomolecules. CDg13 undergoes selective efflux by active ABCG2 transporters, with increased intracellular accumulation following ABCG2 inhibition or knockdown. CDg13 isolates and enriches self-renewable neural stem/progenitor cells from embryonic mouse brain tissue, stains neural stem/progenitor cells brightly, and sorts ABCG2low cell populations from heterogeneous populations[1]. (Ex/Em = 520/553 nm)

In Vitro

CDg13 (1 μM; 1 h) selectively stains live mouse NS5 (NSPC) cells with bright fluorescence, while showing minimal staining of MEF, mESC, and differentiated NS5 cells[1].
CDg13 (1 μM; 1 h) fluorescence in D-NS5 cells is inversely correlated with Abcg2 expression, as demonstrated by enhanced fluorescence in Abcg2-knockdown cells stained with it[1].
CDg13 (1-10 μM; 48 h-7days) has very low toxicity to live mouse NSPCs[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CDg13 Related Antibodies

Immunofluorescence[1]

Cell Line: mouse embryonic fibroblasts (MEF), mouse embryonic stem cells (mESC), mouse NSPC line (NS5), differentiated NS5 (D-NS5)
Concentration: 1 μM
Incubation Time: 1 h
Result: Showed bright fluorescence in NS5 cells, with significantly lower fluorescence in MEF, mESC, and D-NS5 cells, as visualized by fluorescence microscopy and confirmed by flow cytometry histograms.

Cell Cytotoxicity Assay[1]

Cell Line: mouse NSPCs (NS5 cells)
Concentration: 1, 10 μM
Incubation Time: 48 h; 7 days;
Result: Showed no cell toxicity after incubation with 10 μM for 48 h.
Showed no effect on neurosphere size after incubation with 1 μM for 7 days.
Molecular Weight

568.53

Formula

C32H35Cl2NO4
CAS No.
SMILES

CCCCCCN(C(C1=CC=C(C=C1)C2=C3C=C(C(C=C3OC4=C2C=C(C(O)=C4)Cl)=O)Cl)=O)CCCCCC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

CDg13 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CDg131451048-96-6CDg 13CDg-13Fluorescent DyeBCRPBreast cancer resistance proteinABCG2endoplasmic reticulumRPMI-8226 cellsAbcg2embryonic mouse brain tissueneural stem/progenitor cellsMEF cellsKB/ABCG2 cellsmESC cellsNS5 (NSPC) cellsInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
CDg13
Cat. No.:
HY-D3179
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.