2. Academic Validation
  3. Lithospermum erythrorhizon extract inhibits Der p2-induced inflammatory response through alleviation of thymic stromal lymphopoietin, nuclear factor Kappa B, and inflammasome expression in human bronchial epithelial cells

Lithospermum erythrorhizon extract inhibits Der p2-induced inflammatory response through alleviation of thymic stromal lymphopoietin, nuclear factor Kappa B, and inflammasome expression in human bronchial epithelial cells

  • J Ethnopharmacol. 2017 Apr 6:201:1-8. doi: 10.1016/j.jep.2017.02.038.
Chung-Yang Yen 1,  Wen-Dee Chiang 2,  Shang-Yong Liu 3,  Kun-Teng Wang 4,  En-Chih Liao 5,  Ching-Liang Hsieh 6
Affiliations

Affiliations

  • 1 Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, Taichung 40402, Taiwan; Department of Dermatology, Taichung Veterans General Hospital, Taichung 40705, Taiwan. Electronic address: [email protected].
  • 2 Department of Food Science, Tunghai University, Taichung 40704, Taiwan. Electronic address: [email protected].
  • 3 Department of Food Science, Tunghai University, Taichung 40704, Taiwan. Electronic address: [email protected].
  • 4 Brion Research Institute, New Taipei City 23143, Taiwan. Electronic address: [email protected].
  • 5 Department of Medicine, Mackay Medical College, New Taipei City 25245, Taiwan. Electronic address: [email protected].
  • 6 Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, Taichung 40402, Taiwan; Department of Chinese Medicine, China Medical University Hospital, Taichung 40447, Taiwan; Graduate Institute of Acupuncture Science, China Medical University, Taichung 40402, Taiwan; Research Center for Chinese Medicine and Acupuncture, China Medical University, Taichung 40402, Taiwan. Electronic address: [email protected].
PMID: 28254482 DOI: 10.1016/j.jep.2017.02.038
Abstract

Ethnopharmacological relevance: Lithospermum erythrorhizon (LE) and Angelica sinensis (AS), widely used in several folk medicine for wound, pus discharge and dermatitis for the history of several hundred years in Asian countries.

Aim of study: To investigate the therapeutic effect of LE and AS on Der p2-induced inflammatory response in human bronchial epithelial (BEAS-2B) cells.

Methods: The effects of Der p2 stimulation on thymic stromal lymphopoietin (TSLP), the nuclear factor kappa B (NF-κB) pathway, the inflammasome (specifically, the Apoptosis speck-like protein [ASC] and NOD-like Receptor 3 [NLRP3]), Caspase-1 and the signal transducer and activator of transcription (STAT) 3 pathway were evaluated in the human bronchial epithelial (BEAS-2B) cells.

Results: The results indicated that LE, AS, and LE+AS reduced TSLP, I kappa B kinase-α, and NLRP3 levels; LE and AS reduced Caspase-1; LE and LE+AS also reduced NF-κB p50, NF-κB p65, ASC, and STAT3 levels.

Conclusion: Both LE and AS aqueous extracts exert anti-inflammatory effects in Der p2-stimulated BEAS-2B cells. These effects may involve multiple mechanisms, including the inhibition of TSLP production as well as the suppression of IKKα, Caspase-1 and NLRP3; however, additional studies are warranted to elucidate the underlying mechanisms.

Keywords

Angelica sinensis; Inflammasome; Lithospermum erythrorhizon; NF-κB; Signal transducer and activator of transcription 3; Thymic Stromal Lymphopoietin.

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