2. Academic Validation
  3. Involvement of two-pore channels in hydrogen peroxide-induced increase in the level of calcium ions in the cytoplasm of human umbilical vein endothelial cells

Involvement of two-pore channels in hydrogen peroxide-induced increase in the level of calcium ions in the cytoplasm of human umbilical vein endothelial cells

  • Dokl Biochem Biophys. 2017 May;474(1):209-212. doi: 10.1134/S1607672917030152.
P V Avdonin 1,  A D Nadeev 2 3,  E B Tsitrin 4,  A A Tsitrina 4,  P P Avdonin 4,  G Yu Mironova 4,  I L Zharkikh 5,  N V Goncharov 3 6
Affiliations

Affiliations

  • 1 Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, 119334, Russia. [email protected].
  • 2 Institute of Biophysics, Pushchino Branch, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290, Russia.
  • 3 Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, 194223, Russia.
  • 4 Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, 119334, Russia.
  • 5 Research Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow, 125315, Russia.
  • 6 Research Institute of Hygiene, Occupational Pathology, and Human Ecology, Federal Biomedical Agency of Russia, Kapitolovo, Leningrad oblast, 188663, Russia.
PMID: 28726088 DOI: 10.1134/S1607672917030152
Abstract

Hydrogen peroxide at concentrations below cytotoxic ones causes an increase in the cytoplasmic calcium concentration in human umbilical vein endothelial cells as a result of calcium release from intracellular stores. Two-pore Calcium Channel blocker trans-NED19 partially suppresses the increase in the level of calcium ions in the cells in response to the addition of hydrogen peroxide. The staining of endothelial cells with the fluorescent stereoisomer cis-NED19 and LysoTracker confirmed the localization of two-pore calcium channels in lysosomes and endolysosomal vesicles.

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