2. Academic Validation
  3. Antibiofilm potential of synthetic 2-amino-5-chlorobenzophenone Schiff bases and its confirmation through fluorescence microscopy

Antibiofilm potential of synthetic 2-amino-5-chlorobenzophenone Schiff bases and its confirmation through fluorescence microscopy

  • Microb Pathog. 2017 Sep:110:497-506. doi: 10.1016/j.micpath.2017.07.040.
Arshia 1,  Anum Khalid Khan 2,  Khalid Mohammed Khan 3,  Ayaz Ahmed 2,  Muhammad Taha 4,  Shahnaz Perveen 5
Affiliations

Affiliations

  • 1 H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan.
  • 2 Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan.
  • 3 H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan. Electronic address: [email protected].
  • 4 Department of Clinical Pharmacy, Institute for Research and Medical Consultations (IRMC), University of Dammam, Dammam 31441, Saudi Arabia.
  • 5 PCSIR Laboratories Complex, Karachi, Shahrah-e-Dr. Salimuzzaman Siddiqui, Karachi, 75280, Pakistan.
PMID: 28755874 DOI: 10.1016/j.micpath.2017.07.040
Abstract

Antibacterial/antibiofilm potential of microwave-assisted synthetic thirty-three 2-amino-5-chloro benzophenone Schiff Bases have been carried out against four Bacterial strains i.e. Klebsiella pneumoniae, Proteus mirabilis, Staphylococcus aureus and Streptococcus mutans. Among them compounds 5, 6, 8, 9, 14, 16, 22, 24, 26, and 30-32 showed antibiofilm activities against isolates at less than 100 μg/ml concentrations. These compounds showed enhanced antibiofilm activity against S. aureus as compared to cefixime used as control. However, remaining compounds were found to be active but at higher concentration. Fluorescence microscopy has been employed for confirmation of antibiofilm results. The structures of all synthetic molecules have been characterized on the basis of spectroscopic techniques including 1H NMR, 13C NMR, EI-MS, HREI-MS, and IR spectroscopy and their structure-activity relationship have been established.

Keywords

2-Amino-5-chlorobenzophenone Schiff bases; Antibiofilm; Fluorescence microscopy; Klebsiella pneumonia; Microwave-assisted synthesis; Proteus mirabilis; Staphylococcus aureus; Streptococcus mutans.

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