2. Academic Validation
  3. Kaempferol-3-o-β-d-glucuronate exhibit potential anti-inflammatory effect in LPS stimulated RAW 264.7 cells and mice model

Kaempferol-3-o-β-d-glucuronate exhibit potential anti-inflammatory effect in LPS stimulated RAW 264.7 cells and mice model

  • Int Immunopharmacol. 2018 Apr;57:62-71. doi: 10.1016/j.intimp.2018.01.041.
Vidushi Khajuria 1,  Shilpa Gupta 1,  Neha Sharma 2,  Harshita Tiwari 3,  Subhash Bhardwaj 4,  Prabhu Dutt 2,  Naresh Satti 2,  Amit Nargotra 5,  Asha Bhagat 6,  Zabeer Ahmed 7
Affiliations

Affiliations

  • 1 Academy of Scientific and Innovative Research, CSIR-Indian Institute of Integrative Medicines, Jammu Tawi, Jammu and Kashmir, India; Inflammation Pharmacology Division, CSIR-Indian Institute of Integrative Medicine, Jammu Tawi, Jammu and Kashmir, India.
  • 2 Natural Product Chemistry, CSIR-Indian Institute of Integrative Medicines, Jammu Tawi, Jammu and Kashmir, India.
  • 3 Academy of Scientific and Innovative Research, CSIR-Indian Institute of Integrative Medicines, Jammu Tawi, Jammu and Kashmir, India; Discovery Informatics Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu 180001, India.
  • 4 Department of Pathology, Government Medical College, Jammu Tawi, Jammu and Kashmir, India.
  • 5 Discovery Informatics Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu 180001, India.
  • 6 Inflammation Pharmacology Division, CSIR-Indian Institute of Integrative Medicine, Jammu Tawi, Jammu and Kashmir, India.
  • 7 Academy of Scientific and Innovative Research, CSIR-Indian Institute of Integrative Medicines, Jammu Tawi, Jammu and Kashmir, India. Electronic address: [email protected].
PMID: 29475097 DOI: 10.1016/j.intimp.2018.01.041
Abstract

Kaempferol-3-O-β-d-glucuronide (K3G) having various pharmacological effects was explored for its anti-inflammatory effect in LPS induced RAW 264.7 cells and mice model. K3G significantly inhibited various pro-inflammatory mediators like IL-1β, NO, PGE2, and LTB4. It upregulated the secretion of anti-inflammatory cytokine IL-10. K3G is found to reduce inflammation when studied for parameters like phagocytic index, carrageenan induced paw edema in mice and organ weight. It reduced inflammation in a dose dependent manner both in-vitro and in-vivo. Further molecular insights into the study reveal that K3G blocks the phosphorylation of NF-kB which is key regulator of inflammation, thereby exhibiting anti-inflammatory potential. Hence, this study permits further investigation to develop K3G as anti-inflammatory drug.

Keywords

Anti-inflammatory; Histopathology; Pathway elucidation; Proinflammatory.

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