2. Academic Validation
  3. Bach2 regulates aberrant activation of B cell in systemic lupus erythematosus and can be negatively regulated by BCR-ABL/PI3K

Bach2 regulates aberrant activation of B cell in systemic lupus erythematosus and can be negatively regulated by BCR-ABL/PI3K

  • Exp Cell Res. 2018 Apr 1;365(1):138-144. doi: 10.1016/j.yexcr.2018.02.034.
Zhengwei Zhu 1,  Chao Yang 1,  Leilei Wen 1,  Lu Liu 1,  Xianbo Zuo 1,  Fusheng Zhou 1,  Jinping Gao 1,  Xiaodong Zheng 1,  Yinjuan Shi 1,  Caihong Zhu 1,  Bo Liang 1,  Xianyong Yin 1,  Wenjun Wang 1,  Hui Cheng 1,  Songke Shen 1,  Xianfa Tang 1,  Huayang Tang 1,  Liangdan Sun 1,  Anping Zhang 1,  Sen Yang 1,  Yong Cui 2,  Xuejun Zhang 1,  Yujun Sheng 3
Affiliations

Affiliations

  • 1 Institute of Dermatology and Department of Dermatology, the First Affiliated Hospital, Anhui Medical University, No. 81 Meishan Road, Hefei, Anhui 230032, China; Key Laboratory of Dermatology, Anhui Medical University, Ministry of Education, Hefei, Anhui 230032, China.
  • 2 Department of Dermatology, China-Japan Friendship Hospital, East Street Cherry Park, Chaoyang District, Beijing 100029, China.
  • 3 Institute of Dermatology and Department of Dermatology, the First Affiliated Hospital, Anhui Medical University, No. 81 Meishan Road, Hefei, Anhui 230032, China; Key Laboratory of Dermatology, Anhui Medical University, Ministry of Education, Hefei, Anhui 230032, China. Electronic address: [email protected].
PMID: 29501569 DOI: 10.1016/j.yexcr.2018.02.034
Abstract

Objective: This study was aimed to explore the effect of Bach2 on B cells in systemic lupus erythematosus (SLE), as well as the underlying mechanisms.

Methods: Expression of Bach2, phosphorylated-Bach2 (p-Bach2), Akt, p-Akt and Bcr-Abl (p210) in B cells isolated from SLE patients and the healthy persons were assessed by Western blot. Immunofluorescence staining was performed to assess the localization of Bach2 in B cells. Enzyme-linked immunosorbent assay (ELISA) was employed to detect IgG produced by B cells. Cell counting kit-8 (CCK-8) and Annexin-V FITC/PI double staining assay were adopted to evaluate cell proliferation and Apoptosis in B cells, respectively.

Results: Compared to the healthy controls, Bach2, p-Akt and p210 were significantly decreased, while nuclear translocation of Bach2, IgG, CD40 and CD86 obviously up-regulated in B cells from SLE patients. Bach2 significantly inhibited the proliferation, promoted Apoptosis of B cells from SLE patients, whereas Bcr-Abl dramatically reversed cell changes induced by Bach2. Besides, Bcr-Abl also inhibited nuclear translocation of Bach2 in B cells from SLE patients. Further, LY294002 treatment had no effect on decreased expression of Bach2 induced by Bcr-Abl, but significantly eliminated BCR-ABL-induced phosphorylation of Bach2 and restored reduced nuclear translocation of Bach2 induced by Bcr-Abl in B cells from SLE.

Conclusions: Bach2 may play a suppressive role in B cells from SLE, and Bcr-Abl may inhibit the nuclear translocation of Bach2 via serine phosphorylation through the PI3K pathway.

Keywords

B cells; BCR-ABL; Bach2; PI3K; Systemic lupus erythematosus (SLE).

Figures
Products
Inhibitors & Agonists
Other Products