2. Academic Validation
  3. Raddeanin A suppresses breast cancer-associated osteolysis through inhibiting osteoclasts and breast cancer cells

Raddeanin A suppresses breast cancer-associated osteolysis through inhibiting osteoclasts and breast cancer cells

  • Cell Death Dis. 2018 Mar 7;9(3):376. doi: 10.1038/s41419-018-0417-0.
Qiang Wang 1,  Jian Mo 1,  Chenchen Zhao 2,  Kangmao Huang 1,  Mingxuan Feng 3,  Wenxin He 1,  Jiying Wang 1,  Shuai Chen 1,  Zi'ang Xie 1,  Jianjun Ma 4,  Shunwu Fan 5
Affiliations

Affiliations

  • 1 Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Sir Run Run Shaw Institute of Clinical Medicine of Zhejiang University, 3# East Qingchun Road, Hangzhou, Zhejiang Province, 310016, China.
  • 2 Department of Orthopedic Surgery, the Second Affiliated Hospital, Medical College of Zhejiang University, 88#Jiefang Road, Hangzhou, Zhejiang Province, 310016, China.
  • 3 Orthopaedic Department, Taizhou Central Hospital, Affiliate Hospital Of Taizhou University, Taizhou, Zhejiang Province, 318000, China.
  • 4 Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Sir Run Run Shaw Institute of Clinical Medicine of Zhejiang University, 3# East Qingchun Road, Hangzhou, Zhejiang Province, 310016, China. [email protected].
  • 5 Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Sir Run Run Shaw Institute of Clinical Medicine of Zhejiang University, 3# East Qingchun Road, Hangzhou, Zhejiang Province, 310016, China. [email protected].
PMID: 29515110 DOI: 10.1038/s41419-018-0417-0
Abstract

Bone metastasis is a severe complication of advanced breast Cancer, resulting in osteolysis and increased mortality in patients. Raddeanin A (RA), isolated from traditional Chinese herbs, is an oleanane-type triterpenoid saponin with Anticancer potential. In this study, we investigated the effects of RA in breast cancer-induced osteolysis and elucidated the possible mechanisms involved in this process. We first verified that RA could suppress osteoclast formation and bone resorption in vitro. Next, we confirmed that RA suppressed Ti-particle-induced osteolysis in a mouse calvarial model, possibly through inhibition of the Src/Akt signaling pathway. A breast cancer-induced osteolysis mouse model further revealed the positive protective effects of RA by micro-computed tomography and histology. Finally, we demonstrated that RA inhibited invasion and Akt/mammalian target of rapamycin signaling and induced Apoptosis in MDA-MB-231 cells. These results indicate that RA is an effective inhibitor of breast cancer-induced osteolysis.

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