2. Academic Validation
  3. Analysis of 10 β-agonists in pork meat using automated dispersive pipette extraction and LC-MS/MS

Analysis of 10 β-agonists in pork meat using automated dispersive pipette extraction and LC-MS/MS

  • J Chromatogr B Analyt Technol Biomed Life Sci. 2018 May 1;1084:64-68. doi: 10.1016/j.jchromb.2018.03.026.
Kaylee R Mastrianni 1,  Kalmorat Metavarayuth 2,  William E Brewer 1,  Qian Wang 3
Affiliations

Affiliations

  • 1 DPX Technologies, LLC, 151 Powell Rd, Suite 116,Columbia, SC 29203, United States.
  • 2 MicroSep, LLC, 2411 Monroe Street, Columbia, SC 29205, United States; Department of Chemistry and Biochemistry, University of South Carolina, 631 Sumter Street, Columbia, SC 29208, United States.
  • 3 Department of Chemistry and Biochemistry, University of South Carolina, 631 Sumter Street, Columbia, SC 29208, United States. Electronic address: [email protected].
PMID: 29571118 DOI: 10.1016/j.jchromb.2018.03.026
Abstract

An analytical procedure for the analysis of 10 β-adrenergic agonists (cimaterol, terbutaline, salbutamol, isoxsuprine, ractopamine, cimbuterol, clenbuterol, brombuterol, mabuterol and mapenterol) in pork meat was developed and validated using LC-MS/MS. An automated dispersive pipette extraction (DPX) was employed on a Hamilton Microlab® NIMBUS96® platform to extract the analytes of interest prior to LC-MS/MS analysis. The extraction time was <20 min with a total LC-MS/MS run time of 9.6 min. The method was fully validated in accordance with the international guidelines (European Commission Decision 2002/657/EC and National Standards of People's Republic of China, GB/T 22286-2008) for limit of detection, limit of quantitation, carryover, extraction efficiency, matrix effects, linearity, and within and between-run precision. The proposed method can be successfully used in the routine determination of 10 β-adrenergic agonists in pork and as a potential solution for compliance monitoring in regulatory laboratories.

Keywords

Dispersive pipette extraction (DPX); Extraction; LC-MS/MS; β-adrenergic agonist.

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