2. Academic Validation
  3. Safety assessment of sodium acetate, sodium diacetate and potassium sorbate food additives

Safety assessment of sodium acetate, sodium diacetate and potassium sorbate food additives

  • Food Chem. 2018 Aug 15:257:211-215. doi: 10.1016/j.foodchem.2018.03.020.
Hossein Mohammadzadeh-Aghdash 1,  Yousef Sohrabi 1,  Ali Mohammadi 2,  Dariush Shanehbandi 2,  Parvin Dehghan 3,  Jafar Ezzati Nazhad Dolatabadi 4
Affiliations

Affiliations

  • 1 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Food Science and Technology, Faculty of Nutrition and Food Sciences, Nutrition Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
  • 2 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
  • 3 Department of Food Science and Technology, Faculty of Nutrition and Food Sciences, Nutrition Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: [email protected].
  • 4 Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: [email protected].
PMID: 29622200 DOI: 10.1016/j.foodchem.2018.03.020
Abstract

Cytotoxicity and genotoxicity of sodium acetate (SA), sodium diacetate (SDA), and potassium sorbate (PS) was tested on Human Umbilical Vein Endothelial Cells (HUVEC). Cytotoxicity was investigated by MTT assay and flow cytometry analysis, while genotoxicity was evaluated using DNA fragmentation and DAPI staining assays. The growth of treated HUVECs with various concentrations of SA, SDA and PS decreased in a dose-and time-dependent manner. The IC50 of 487.71, 485.82 and 659.96 µM after 24 h and IC50 of 232.05, 190.19 and 123.95 µM after 48 h of treatment were attained for SA, SDA and PS, respectively. Flow cytometry analysis showed that early and late Apoptosis percentage in treated cells was not considerable. Also neither considerable DNA fragmentation nor DNA smear was observed using DAPI staining and DNA ladder assays. Overall, it can be concluded that the aforementioned food additives can be used as safe additives at low concentration in food industry.

Keywords

Cytotoxicity; Food additives; Genotoxicity; HUVECs; Potassium sorbate; Sodium acetate; Sodium diacetate.

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