2. Academic Validation
  3. Chemogenetic control of gene expression and cell signaling with antiviral drugs

Chemogenetic control of gene expression and cell signaling with antiviral drugs

  • Nat Methods. 2018 Jul;15(7):519-522. doi: 10.1038/s41592-018-0042-y.
Elliot P Tague 1,  Hannah L Dotson 1,  Shannon N Tunney 1,  D Christopher Sloas 1,  John T Ngo 2
Affiliations

Affiliations

  • 1 Department of Biomedical Engineering and Biological Design Center, Boston University, Boston, MA, USA.
  • 2 Department of Biomedical Engineering and Biological Design Center, Boston University, Boston, MA, USA. [email protected].
PMID: 29967495 DOI: 10.1038/s41592-018-0042-y
Abstract

We developed a method in which the NS3 cis-protease from hepatitis C virus can be used as a ligand-inducible connection to control the function and localization of engineered proteins in mammalian cells. To demonstrate the versatility of this approach, we designed drug-sensitive transcription factors and transmembrane signaling proteins, the activities of which can be tightly and reversibly controlled through the use of clinically tested antiviral Protease Inhibitors.

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