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  2. Thiol-Disulfide Exchange Reaction for Cellular Glutathione Detection with Surface-Enhanced Raman Scattering

Thiol-Disulfide Exchange Reaction for Cellular Glutathione Detection with Surface-Enhanced Raman Scattering

  • Anal Chem. 2018 Oct 2;90(19):11333-11339. doi: 10.1021/acs.analchem.8b01974.
Chenghua Wei 1 Xiao Liu 1 Yun Gao 1 Yiping Wu 1 Xiaoyu Guo 1 Ye Ying 1 Ying Wen 1 Haifeng Yang 1
Affiliations

Affiliation

  • 1 The Education Ministry Key Lab of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors and Department of Chemistry , Shanghai Normal University , Shanghai 200234 , China.
Abstract

Abnormal glutathione (GSH) levels are related to several diseases, including Cancer. In this paper, surface-enhanced Raman spectroscopy (SERS) based on a 2-(pyridyldithio)ethylamine (PDEA)-modified AgNPs@Si wafer is proposed for intracellular GSH detection. PDEA plays multifunctional roles in the method, including the participation in the thiol-disulfide exchange reaction and the contribution of a great SERS signal reporter. With the addition of GSH, the disulfide bond of PDEA will be broken, releasing the pyridine ring in PDEA and resulting in a signal-off SERS response. The developed specific reaction-based SERS assay can detect GSH levels as low as 2.5 × 10-7 M. Subsequently, we employed this method to evaluate cellular GSH levels and found that the levels in Cancer cells are higher than those in normal cells. The PDEA-modified AgNPs@Si-based SERS protocol demonstrates good selectivity and high sensitivity, as well as robustness, which is suitable to evaluate cellular GSH levels.

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