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  3. Optical molecular imaging of lysyl oxidase activity - detection of active fibrogenesis in human lung tissue

Optical molecular imaging of lysyl oxidase activity - detection of active fibrogenesis in human lung tissue

  • Chem Sci. 2015 Aug 1;6(8):4946-4953. doi: 10.1039/c5sc01258a.
Tashfeen Aslam 1,  Amy Miele 2,  Sunay V Chankeshwara 1,  Alicia Megia-Fernandez 1,  Chesney Michels 2,  Ahsan R Akram 2,  Neil McDonald 2,  Nik Hirani 2,  Chris Haslett 2,  Mark Bradley 1,  Kevin Dhaliwal 2
Affiliations

Affiliations

  • 1 School of Chemistry , EaStChem , University of Edinburgh , Joseph Black Building, West Mains Road , Edinburgh , EH9 3FJ , UK . Email: [email protected].
  • 2 Pulmonary Optical Molecular Imaging Group , MRC/Centre of Inflammation Research , Queen's Medical Research Institute , University of Edinburgh , 47 Little France Crescent , EH16 4TJ , Edinburgh , UK . Email: [email protected].
PMID: 30155003 DOI: 10.1039/c5sc01258a
Abstract

Aberrant fibrogenesis is a feature of many diseases in multiple organ systems. The Lysyl Oxidase family of Enzymes are central to tissue homeostasis and elevated Lysyl Oxidase activity is implicated in fibroproliferation as well as in Cancer stroma. We have synthesised a novel fluorogenic reporter for monitoring Lysyl Oxidase activity that generates a 3-5 fold increase in fluorescence following probe activation in ventilating fibrotic ex vivo asinine lung and ex vivo human lung tissue. The probe termed "oLOX" can provide real-time measurement of Lysyl Oxidase activity in a number of biological settings and is tractable from an in vitro setting to man.

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