2. Academic Validation
  3. Structural characterization and in vitro hypoglycemic activity of a glucan from Euryale ferox Salisb. seeds

Structural characterization and in vitro hypoglycemic activity of a glucan from Euryale ferox Salisb. seeds

  • Carbohydr Polym. 2019 Apr 1;209:363-371. doi: 10.1016/j.carbpol.2019.01.044.
Wen-Na Zhang 1,  Ri-Na Su 2,  Li-Li Gong 2,  Wei-Wei Yang 2,  Juan Chen 2,  Rui Yang 2,  Ya Wang 2,  Wen-Juan Pan 1,  Yong-Ming Lu 3,  Yan Chen 4
Affiliations

Affiliations

  • 1 School of Life Sciences, Anhui University, Hefei 230601, Anhui, PR China; Key Laboratory of Anhui Ecological Engineering and Biotechnology, Hefei 230601, Anhui, PR China.
  • 2 School of Life Sciences, Anhui University, Hefei 230601, Anhui, PR China.
  • 3 School of Life Sciences, Anhui University, Hefei 230601, Anhui, PR China; Key Laboratory of Anhui Ecological Engineering and Biotechnology, Hefei 230601, Anhui, PR China; Key Laboratory of Modern Biomanufacturing of Anhui Province, Hefei 230601, Anhui, PR China.
  • 4 School of Life Sciences, Anhui University, Hefei 230601, Anhui, PR China; Key Laboratory of Anhui Ecological Engineering and Biotechnology, Hefei 230601, Anhui, PR China; Key Laboratory of Modern Biomanufacturing of Anhui Province, Hefei 230601, Anhui, PR China. Electronic address: [email protected].
PMID: 30732819 DOI: 10.1016/j.carbpol.2019.01.044
Abstract

In this research, a polysaccharide fraction (EFSP-1) was obtained from the seeds of Euryale ferox Salisb. by DEAE sepharose FF and Superdex™ 75 gel chromatography. The average molecular weight (Mw) of EFSP-1 was 8.75 kDa. Monosaccharides composition analysis indicated that EFSP-1 was a glucan. The structure of EFSP-1 was characterized by analysis of FT-IR, GC-MS and NMR, which indicated that the backbone of EFSP-1 was mainly composed of (1→4)-α-D-Glcp with branches substituted at O-6 and terminated with T-α-D-Glcp. Moreover, the hypoglycemic effect of EFSP-1 was investigated by establishing Insulin resistance HepG2 and 3T3-L1 cells. The results showed that EFSP-1 could increase glucose consumption by up-regulating the expression of GLUT-4 via activating PI3K/Akt signal pathway in IR cells. Hence, EFSP-1 could be a potential functional food to ameliorate Insulin resistance for diabetes therapy.

Keywords

1-phenyl-3-methyl-5-pyrazolone (PubChem CID: 90474051); 3T3-L1 cells; Acetic anhydride (PubChem CID: 7918); D-arabinose (PubChem CID: 229); D-galactose (PubChem CID: 6036); D-glucose (PubChem CID: 5793); D-mannose (PubChem CID: 18950); Dimethyl sulfoxide (PubChem CID: 679); Euryale feroxseed; Glucan; Glucuronic acid (PubChem CID: 94715); HepG2 cells; Hypoglycemic activity; Insulin resistance; Methyl iodide (PubChem CID: 6328); Pyridine (PubChem CID: 1049); Sodium borohydride (PubChem CID: 4311764); Sodium hydroxide (PubChem CID: 14798); Trifluoroacetic acid (PubChem CID: 6422).

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