2. Academic Validation
  3. Mechanical cues modulate cellular uptake of nanoparticles in cancer via clathrin-mediated and caveolae-mediated endocytosis pathways

Mechanical cues modulate cellular uptake of nanoparticles in cancer via clathrin-mediated and caveolae-mediated endocytosis pathways

  • Nanomedicine (Lond). 2019 Mar;14(5):613-626. doi: 10.2217/nnm-2018-0334.
Xiao Wei 1,  Rui Wei 1,  Guiying Jiang 1,  Yijuan Jia 2,  Hua Lou 1,  Zongyuan Yang 1,  Danfeng Luo 1,  Quanfu Huang 3,  Sen Xu 1,  Xin Yang 1,  Ying Zhou 1,  Xiaoting Li 1,  Teng Ji 1,  Junbo Hu 4,  Ling Xi 1,  Ding Ma 1,  Fei Ye 5,  Qinglei Gao 1
Affiliations

Affiliations

  • 1 Department of Obstetrics & Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, People's Republic of China.
  • 2 Department of Obstetrics & Gynecology, Wuhan First Hospital, Wuhan, 430022, China.
  • 3 Department of Thoracic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, People's Republic of China.
  • 4 Cancer Biology Research Center (Key Laboratory of the Ministry of Education), Tongji Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, People's Republic of China.
  • 5 Department of Neurosurgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, People's Republic of China.
PMID: 30816057 DOI: 10.2217/nnm-2018-0334
Abstract

Aim: To investigate the influence of tissue mechanics on the cellular uptake efficiency of nanoparticles (NPs) in Cancer.

Materials & methods: Collagen-coated polyacrylamide gels were prepared as model substrates. Coumarin 6-loaded poly(lactic-co-glycolic) acid micelles (C6-NPs) were prepared to investigate the cellular uptake of NPs.

Results: We demonstrated that substrate stiffness modulated the cellular uptake of NPs of Cancer. Mechanistically, mechanical cues exerted influence on the clathrin-mediated endocytosis and caveolae-mediated endocytosis pathways, which mediated stiffness-regulated cellular uptake of NPs.

Conclusion: Our findings shed LIGHT on the regulatory role of the mechanical cues on the cellular uptake of NPs and will facilitate the selection of clinical patients who might benefit from a given nanotherapy.

Keywords

cancer; cellular uptake; endocytic pathways; matrix stiffness; nanoparticle.

Figures
Products