1. Cytoskeleton
  2. Myosin
  3. (-)-Blebbistatin

(-)-Blebbistatin (Synonyms: (S)-(-)-Blebbistatin)

Cat. No.: HY-13441 Purity: 99.42%
Handling Instructions

(-)-Blébbistatine est un inhibiteur de l'activité ATPase de la myosine II non musculaire.

(-)-Blebbistatin ist ein Inhibitor der ATPase-Aktivität von Nicht-Muskel-Myosin II.

(-)-Blebbistatin is a selective inhibitor of the ATPase activity of non-muscle myosin II.

For research use only. We do not sell to patients.

(-)-Blebbistatin Chemical Structure

(-)-Blebbistatin Chemical Structure

CAS No. : 856925-71-8

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5 mg USD 72 In-stock
Estimated Time of Arrival: December 31
10 mg USD 120 In-stock
Estimated Time of Arrival: December 31
50 mg USD 420 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of (-)-Blebbistatin:

Top Publications Citing Use of Products

    (-)-Blebbistatin purchased from MCE. Usage Cited in: Adv Sci. 2020 Jun 17;7(15):1903583.

    Immunofluorescence imaging of LNCaP cells treated with the Cytoskeleton B (CB), Nocodazole (NO), PF-573288 (PF), Y-27632 (Y), and (-)-Blebbistatin ((-) Bl). After the cells are cultured on different substrates for 3 days, inhibitors are added and incubated for 24 h.

    (-)-Blebbistatin purchased from MCE. Usage Cited in: Adv Sci. 2020 Jun 17;7(15):1903583.

    Expression of CTC cluster-related (plakoglobin and CD44) and EMT-related (vimentin and E-cadherin, 4 days) proteins in LNCaP cells grown on different substrates after treatment with different inhibitors (Cytoskeleton B (CB), Nocodazole (NO), PF-573288 (PF), Y-27632 (Y), and (-)-Blebbistatin ((-) Bl)).
    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review


    (-)-Blebbistatin is a selective inhibitor of the ATPase activity of non-muscle myosin II[1][2].

    IC50 & Target

    IC50: 0.5 to 5 μM (myosin II)[1]

    In Vitro

    Blebbistatin potently inhibits several striated muscle myosins as well as vertebrate nonmuscle myosin IIA and IIB with IC50 values ranging from 0.5 to 5 μM. Smooth muscle myosin is only poorly inhibited (IC50=80 μM)[1]. Blebbistatin does not compete with nucleotide binding to the skeletal muscle myosin subfragment-1. The inhibitor preferentially binds to the ATPase intermediate with ADP and phosphate bound at the active site, and it slows down phosphate release. It blocks the myosin heads in a products complex with low actin affinity[2]. In culture-activated hepatic stellate cells, blebbistatin is found to change both cell morphology and function. Stellate cells become smaller, acquire a dendritic morphology and have less myosin IIA-containing stress fibres and vinculin-containing focal adhesions. Blebbistatin impairs silicone wrinkle formation, reduces collagen gel contraction and blocks endothelin-1-induced intracellular Ca2+ release. It promotes wound-induced cell migration[3].

    In Vivo

    Blebbistatin dose-dependently and completely relax both KCl- and carbachol-induced rat detrusor and endothelin-1-induced human bladder contraction. Pre-incubation with 10 μM blebbistatin attenuates carbachol responsiveness by 65% while blocking electrical field stimulation-induced bladder contraction reaching 50% inhibition at 32 Hz[4].

    Molecular Weight




    CAS No.



    CC1=CC=C(N=C(N(C2=CC=CC=C2)CC3)[[email protected]]3(O)C4=O)C4=C1


    Room temperature in continental US; may vary elsewhere.


    4°C, stored under nitrogen

    *The compound is unstable in solutions, freshly prepared is recommended.

    Solvent & Solubility
    In Vitro: 

    DMSO : 10 mg/mL (34.21 mM; Need ultrasonic)

    H2O : < 0.1 mg/mL (insoluble)

    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.4208 mL 17.1040 mL 34.2079 mL
    5 mM 0.6842 mL 3.4208 mL 6.8416 mL
    10 mM 0.3421 mL 1.7104 mL 3.4208 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 1 mg/mL (3.42 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: 1 mg/mL (3.42 mM); Suspended solution; Need ultrasonic

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 1 mg/mL (3.42 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    Cell Assay

    Freshly isolated HSCs are replated on 96-well plate. At day 3, medium is replaced by serum-free medium and cells are starved overnight, treated with or without blebbistatin (25 μM) for 2 h followed by stimulation with platelet-derived growth factor-BB (20 ng/mL). After an overnight incubation, the WST-1 cell proliferation assay are performed[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.


    Purity: 99.42%

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