1. Cytoskeleton
  2. Myosin

(-)-Blebbistatin (Synonyms: (S)-(-)-Blebbistatin)

Cat. No.: HY-13441 Purity: 99.47%
Data Sheet SDS Handling Instructions

(-)-Blebbistatin is an S enantiomer of blebbistatin. Blebbistatin is a potent and selective myosin II inhibitor with IC50 values ranging from 0.5 to 5 μM.

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(-)-Blebbistatin Chemical Structure

(-)-Blebbistatin Chemical Structure

CAS No. : 856925-71-8

Size Price Stock Quantity
10 mM * 1 mL in DMSO $66 In-stock
5 mg $60 In-stock
10 mg $100 In-stock
50 mg $350 In-stock
100 mg   Get quote  
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Description

(-)-Blebbistatin is an S enantiomer of blebbistatin. Blebbistatin is a potent and selective myosin II inhibitor with IC50 values ranging from 0.5 to 5 μM.

IC50 & Target

IC50: 0.5 to 5 μM (myosin II)[1]

In Vitro

Blebbistatin potently inhibits several striated muscle myosins as well as vertebrate nonmuscle myosin IIA and IIB with IC50 values ranging from 0.5 to 5 μM. Smooth muscle myosin is only poorly inhibited (IC50=80 μM)[1]. Blebbistatin does not compete with nucleotide binding to the skeletal muscle myosin subfragment-1. The inhibitor preferentially binds to the ATPase intermediate with ADP and phosphate bound at the active site, and it slows down phosphate release. It blocks the myosin heads in a products complex with low actin affinity[2]. In culture-activated hepatic stellate cells, blebbistatin is found to change both cell morphology and function. Stellate cells become smaller, acquire a dendritic morphology and have less myosin IIA-containing stress fibres and vinculin-containing focal adhesions. Blebbistatin impairs silicone wrinkle formation, reduces collagen gel contraction and blocks endothelin-1-induced intracellular Ca2+ release. It promotes wound-induced cell migration[3].

In Vivo

Blebbistatin dose-dependently and completely relax both KCl- and carbachol-induced rat detrusor and endothelin-1-induced human bladder contraction. Pre-incubation with 10 μM blebbistatin attenuates carbachol responsiveness by 65% while blocking electrical field stimulation-induced bladder contraction reaching 50% inhibition at 32 Hz[4].

References
Preparing Stock Solutions
Concentration Volume (DMSO) Mass 1 mg 5 mg 10 mg
1 mM 3.4208 mL 17.1040 mL 34.2079 mL
5 mM 0.6842 mL 3.4208 mL 6.8416 mL
10 mM 0.3421 mL 1.7104 mL 3.4208 mL
Cell Assay
[3]

Freshly isolated HSCs are replated on 96-well plate. At day 3, medium is replaced by serum-free medium and cells are starved overnight, treated with or without blebbistatin (25 μM) for 2 h followed by stimulation with platelet-derived growth factor-BB (20 ng/mL). After an overnight incubation, the WST-1 cell proliferation assay are performed[3]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

292.33

Formula

C₁₈H₁₆N₂O₂

CAS No.

856925-71-8

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: 5.2 mg/mL (Need ultrasonic and warming)

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

Purity: 99.47%

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Product Name:
(-)-Blebbistatin
Cat. No.:
HY-13441
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