2. Academic Validation
  3. Sophoraflavanone G from Sophora flavescens induces apoptosis in triple-negative breast cancer cells

Sophoraflavanone G from Sophora flavescens induces apoptosis in triple-negative breast cancer cells

  • Phytomedicine. 2019 Aug;61:152852. doi: 10.1016/j.phymed.2019.152852.
Wen-Chung Huang 1,  Pei-Yu Gu 2,  Li-Wen Fang 3,  Yu-Ling Huang 4,  Chwan-Fwu Lin 5,  Chian-Jiun Liou 6
Affiliations

Affiliations

  • 1 Graduate Institute of Health Industry Technology, Research Center for Food and Cosmetic Safety, Research Center for Chinese Herbal Medicine, College of Human Ecology, Chang Gung University of Science and Technology, No.261, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33303, Taiwan; Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital, Linkou, Guishan Dist., Taoyuan City 33303, Taiwan.
  • 2 Graduate Institute of Health Industry Technology, Research Center for Food and Cosmetic Safety, Research Center for Chinese Herbal Medicine, College of Human Ecology, Chang Gung University of Science and Technology, No.261, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33303, Taiwan.
  • 3 Department of Nutrition, I-Shou University, No.8, Yida Rd. Yanchao Dist., Kaohsiung City, Taiwan.
  • 4 National Research Institute of Chinese Medicine, Ministry of Health and Welfare, No. 155-1, Sec. 2, Li-Nung St., Peitou, Taipei, Taiwan; Department of Cosmetic Science, Research Center for Food and Cosmetic Safety, Research Center for Chinese Herbal Medicine, College of Human Ecology, Chang Gung University of Science and Technology, No.261, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33303, Taiwan.
  • 5 Department of Cosmetic Science, Research Center for Food and Cosmetic Safety, Research Center for Chinese Herbal Medicine, College of Human Ecology, Chang Gung University of Science and Technology, No.261, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33303, Taiwan; Department of Anesthesiology, Chang Gung Memorial Hospital, Linkou, Guishan Dist., Taoyuan City 33303, Taiwan. Electronic address: [email protected].
  • 6 Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital, Linkou, Guishan Dist., Taoyuan City 33303, Taiwan; Department of Nursing, Division of Basic Medical Sciences, Research Center for Chinese Herbal Medicine, and Graduate Institute of Health Industry Technology, Chang Gung University of Science and Technology, No.261, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33303, Taiwan. Electronic address: [email protected].
PMID: 31035052 DOI: 10.1016/j.phymed.2019.152852
Abstract

Background: A compound isolated from Sophora flavescens-sophoraflavanone G (SG)-showed anti-tumor and anti-inflammatory properties. We previously demonstrated that SG promoted Apoptosis in human leukemia HL-60 cells. In the present study, we investigated the effects of SG on Apoptosis in human breast Cancer MDA-MB-231 cells, and explored the underlying molecular mechanisms.

Methods: MDA-MB-231 cells were treated with various SG concentrations, and cell viability was evaluated by MTT assay. Apoptotic signal proteins were detected by western blotting, and cell Apoptosis was assessed using flow cytometry.

Results: Our results demonstrated that SG induced nuclear condensation, DNA fragmentation, Reactive Oxygen Species production, and increased cell Apoptosis in MDA-MB-231 cells. SG also suppressed migration and invasion, likely via blockage of the MAPK pathway. In the apoptotic signaling pathway, SG increased cleaved Caspase-8, Caspase-3, and caspase-9. SG treatment also decreased Bcl-2 and Bcl-xL expression, increased Bax expression, and prompted release of more cytochrome c from mitochondria to the cytoplasm in MDA-MB-231 cells.

Conclusion: Overall, our findings suggest that SG might increase Apoptosis, and decrease migration and invasion, in MDA-MB-231 cells through suppression of a MAPK-related pathway.

Keywords

Apoptosis; Caspase-3; MDA-MB-231 cells; Sophoraflavanone G.

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