2. Academic Validation
  3. Effect of sec-O-glucosylhamaudol on mechanical allodynia in a rat model of postoperative pain

Effect of sec-O-glucosylhamaudol on mechanical allodynia in a rat model of postoperative pain

  • Korean J Pain. 2019 Apr 1;32(2):87-96. doi: 10.3344/kjp.2019.32.2.87.
Gi-Ho Koh 1,  Hyun Song 2,  Sang Hun Kim 2 3,  Myung Ha Yoon 4,  Kyung Joon Lim 2 3,  Seon-Hee Oh 5,  Ki Tae Jung 2 3
Affiliations

Affiliations

  • 1 Department of Anesthesiology and Pain Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
  • 2 Department of Anesthesiology and Pain Medicine, Chosun University Hospital, Gwangju, Korea.
  • 3 Department of Anesthesiology and Pain Medicine, School of Medicine, Chosun University, Gwangju, Korea.
  • 4 Department of Anesthesiology and Pain Medicine, Medical School, Chonnam National University, Gwangju, Korea.
  • 5 School of Medicine, Chosun University, Gwangju, Korea.
PMID: 31091507 DOI: 10.3344/kjp.2019.32.2.87
Abstract

Background: This study was performed in order to examine the effect of intrathecal sec-O-glucosylhamaudol (SOG), an extract from the root of the Peucedanum japonicum Thunb., on incisional pain in a rat model.

Methods: The intrathecal catheter was inserted in male Sprague-Dawley rats (n = 55). The postoperative pain model was made and paw withdrawal thresholds (PWTs) were evaluated. Rats were randomly treated with a vehicle (70% dimethyl sulfoxide) and SOG (10 μg, 30 μg, 100 μg, and 300 μg) intrathecally, and PWT was observed for four hours. Dose-responsiveness and ED50 values were calculated. Naloxone was administered 10 min prior to treatment of SOG 300 μg in order to assess the involvement of SOG with an Opioid Receptor. The protein levels of the δ-opioid receptor, κ-opioid receptor, and μ-opioid receptor (MOR) were analyzed by Western blotting of the spinal cord.

Results: Intrathecal SOG significantly increased PWT in a dose-dependent manner. Maximum effects were achieved at a dose of 300 μg at 60 min after SOG administration, and the maximal possible effect was 85.35% at that time. The medial effective dose of intrathecal SOG was 191.3 μg (95% confidence interval, 102.3-357.8). The antinociceptive effects of SOG (300 μg) were significantly reverted until 60 min by naloxone. The protein levels of MOR were decreased by administration of SOG.

Conclusions: Intrathecal SOG showed a significant antinociceptive effect on the postoperative pain model and reverted by naloxone. The expression of MOR were changed by SOG. The effects of SOG seem to involve the MOR.

Keywords

11-hydroxy-sec-O-glocosylhamaudol; Analgesia; Blotting; Dimethyl sulfoxide; Hyperalgesia; Nociceptive pain; Pain; Rats; Receptors; Spinal cord; opioid; postoperative; western.

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